Dataset Information

DNA methylation shapes seed-borne microbiome and proteome responses during early maize-beneficial bacteria interactions

ABSTRACT: Project Description: The interaction between microorganisms and plants plays a key role in plant development and environmental adaptation. Among these microorganisms, plant growth-promoting bacteria (PGPB) enhance agricultural productivity sustainably. Beyond direct effects on plant physiology, epigenetic modifications such as DNA methylation regulate gene expression and adaptive responses. This study investigates how DNA hypomethylation influences early interactions between maize (Zea mays) and the PGPB Herbaspirillum seropedicae, focusing on plant growth, metabolism, and root microbiome. Treatment with the hypomethylating agent 5-azacytidine (5-azaC) did not affect bacterial growth but induced notable phenotypic changes in maize, particularly in root morphology. Inoculation with H. seropedicae enhanced plant growth across biometric parameters. Microscopy revealed bacterial colonization primarily in root mucilage, with higher bacterial accumulation in 5-azaC-treated roots. Global methylation analysis indicated that H. seropedicae modulates cytosine methylation similarly to 5-azaC, suggesting a role in epigenetic regulation. Gene expression analysis of DNA methylation machinery supports hypomethylation as a driver of plant-microbe interactions. Root microbiome profiling showed that 5-azaC significantly altered microbial composition, whereas bacterial inoculation partially restored it toward control profiles. Proteomic analysis identified 1,818 proteins, highlighting significant shifts in metabolic pathways, especially carbon metabolism and the citric acid cycle. These findings demonstrate that DNA hypomethylation, combined with bacterial interaction, profoundly impacts cellular and metabolic processes, offering new insights into early plant-microbe interactions. This knowledge may contribute to developing sustainable agricultural practices through epigenetic and microbial modulation.

INSTRUMENT(S):

ORGANISM(S): Zea Mays (maize)

TISSUE(S): Plant Cell, Root

SUBMITTER: Clicia Grativol

LAB HEAD: Clicia Grativol Gaspar de Matos

PROVIDER: PXD077098 | Pride | 2026-05-05

REPOSITORIES: Pride

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			Action	DRS
	2024_08_15_Clicia_Milho_A1_B.raw.zip	Raw
	2024_08_15_Clicia_Milho_A2.raw.zip	Raw
	2024_08_15_Clicia_Milho_A3.raw.zip	Raw
	2024_08_15_Clicia_Milho_AB1.raw.zip	Raw
	2024_08_15_Clicia_Milho_AB2.raw.zip	Raw

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Publications

DNA Methylation Shapes Seed-Borne Microbiome and Proteome Responses During Early Maize-Beneficial Bacteria Interactions.

Zocateli Pedro Igor PI Olímpio Geovanna Vitória GV de Almeida Felipe Astolpho FA da Rocha Letícia Oliveira LO de Sena Martins Diego Vinicius DV Sangi Sara S Olivares Fábio Lopes FL de Matos Elyabe Monteiro EM Siqueira Juliana J Viccini Lyderson Facio LF da Paschoa Roberta Pena RP Silveira Vanildo V Grativol Clícia C

Plant, cell & environment 20260422

Microorganism and plant interactions are crucial for development and environmental adaptation. Plant growth promoting bacteria enhance agricultural productivity in a sustainable manner, while epigenetic modifications such as DNA methylation regulate gene expression and adaptive responses. The objective of this study is to determine how DNA hypomethylation influences early interactions between maize (Zea mays) and the endophytic diazotrophic bacterium Herbaspirillum seropedicae, particularly rega ...[more]

PMID: 42017455

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