Cryptococcus Wcp1-mCherry Wcp1-FLAG AP–MS

ABSTRACT: This study aims to identify Wcp1-interacting proteins in Cryptococcus neoformans under different pH conditions. Wcp1 is a WD40–cyclophilin fusion protein implicated in adaptation to host-associated stresses, including CO2, temperature, and acidic pH. Affinity purification coupled with liquid chromatography–tandem mass spectrometry (AP–LC–MS/MS) was performed using Wcp1-mCherry and Wcp1-4×FLAG tagged strains to define the Wcp1 interactome under neutral (pH 7) and acidic (pH 5) conditions.

INSTRUMENT(S):

ORGANISM(S): Fungi Cryptococcus Neoformans H99-ypd_1

DISEASE(S): Disease Free

SUBMITTER: Jin-Tae Choi  

LAB HEAD: Yong-Sun Bahn

PROVIDER: PXD077146 | Pride | 2026-05-16

REPOSITORIES: Pride