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Enhancing the Recovery of Antioxidant Compounds from Microalgae-Cyanobacteria Consortia through Alcalase Hydrolysis: a Focus on Bioactive Peptides

ABSTRACT: Microalgae and cyanobacteria represent an emerging and sustainable source of bioactive compounds for the food, cosmeceutical, and pharmaceutical sectors. In this study, the potential of two microalgae-cyanobacteria consortia, consortium 1 (C1) consisting of Chlorella vulgaris and Arthrospira platensis, and consortium 2 (C2) consisting of Kamptonema sp., Nannochloropsis oculata, Tetraselmis suecica, and Chlorella vulgaris, as a source of bioactive peptides was evaluated. Firstly, protein extraction from both biomasses was optimized by testing different protein solubilization and precipitation pHs, with pH 10 and pH 5 providing the best results in terms of protein recovery in both cases. Selected protein extracts, with protein contents of 28.50 ± 2.69 % (C1) and 8.46 ± 0.45 % (C2), were further hydrolyzed with Alcalase, evaluating the impact of the incubation time on peptide release and the antioxidant capacity of hydrolysates. 1 h of hydrolysis proved to be enough for antioxidant capacity increase. In addition, in silico hydrolysis with Alcalase of the proteins identified in C1 and C2 was evaluated, assessing the potential bioactivity of the peptides produced, more specifically their antioxidant capacity. Our findings demonstrate that both microalgae-cyanobacteria consortia are valuable sources of bioactive compounds with antioxidant capacity, with potential interest as functional ingredients for the food, cosmeceutical, and pharmaceutical industries.

INSTRUMENT(S):

ORGANISM(S): Nannochloropsis Oculata Kamptonema Sp. Chlorella Vulgaris Tetraselmis Suecica

SUBMITTER: Blanca Pardo de Donlebún

LAB HEAD: Blanca Hernández-Ledesma

PROVIDER: PXD077149 | Pride | 2026-05-21

REPOSITORIES: Pride

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			Action	DRS
	20241112_3_PROT2292_1.mgf	Mgf
	Holobiont_2.mzidentml_1_2_0.xml	Mzid
	Holobiont_2.pep.xml	Pepxml
	Holobiont_2.proteins.html	Other
	Holobiont_2.raw	Raw

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Enhancing the Recovery of Antioxidant Compounds from Microalgae-Cyanobacteria Consortia through Alcalase Hydrolysis: a Focus on Bioactive Peptides

Project description:Microalgae and cyanobacteria represent an emerging and sustainable source of bioactive compounds for the food, cosmeceutical, and pharmaceutical sectors. In this study, the potential of two microalgae-cyanobacteria consortia, consortium 1 (C1) consisting of Chlorel-la vulgaris and Arthrospira platensis, and consortium 2 (C2) consisting of Kamptonema sp., Nannochloropsis oculata, Tetraselmis suecica, and Chlorella vulgaris, as a source of bioactive peptides was evaluated. Firstly, protein extraction from both biomasses was optimized by testing different protein solubilization and precipitation pHs, with pH 10 and pH 5 providing the best results in terms of protein recovery in both cases. Selected protein ex-tracts, with protein contents of 28.50 ± 2.69 % (C1) and 8.46 ± 0.45 % (C2), were further hy-drolyzed with Alcalase, evaluating the impact of the incubation time on peptide release and the antioxidant capacity of hydrolysates. 1 h of hydrolysis proved to be enough for antioxidant capacity increase. In addition, in silico hydrolysis with Alcalase of the proteins identified in C1 and C2 was evaluated, assessing the potential bioactivity of the peptides produced, more specifically their antioxidant capacity. Our findings demonstrate that both microalgae-cyanobacteria consortia are valuable sources of bioactive compounds with antioxidant capacity, with potential interest as functional ingredients for the food, cosmeceutical, and pharmaceutical industries.

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