Proteome-Driven Phenotyping of Identified Single Neurons in Intact Brain Tissue by Capillary Electrophoresis–timsTOF Mass Spectrometry Patch Proteomics

ABSTRACT: This project developed an ultrahigh-sensitivity proteomics platform in the discovery setting to profile the proteome of cell bodies (somas) between different types of neurons in the mouse central nervous system. The soma contents of mitral cells, serotonergic neurons, dopaminergic neurons, and parvalbumin neurons in the olfactory bulb, ventral tegmental area, prefrontal cortex, and dorsal raphe nucleus of a mouse were collected using a patch pipette, respectively. A microanalytical capillary electrophoresis (CE) electrospray ionization (ESI) platform was developed and coupled to a trapped ion mobility mass spectrometer (timsTOF Pro, Bruker Daltonics, Billerica, MA). Label-free quantification of the soma proteome revealed systematic differences between the samples, with these differences corresponding to neuronal phenotypes. This work marks the first example for the discovery proteomic classification of single neurons in the mammalian brain.

INSTRUMENT(S):

ORGANISM(S): Mus Musculus (mouse)

TISSUE(S): Brain, Neuron

SUBMITTER: Peter Nemes  

LAB HEAD: Peter Nemes

PROVIDER: PXD077382 | Pride | 2026-09-28

REPOSITORIES: Pride