Proteomics

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QXL-MS of insulin resistance in mouse skeletal muscle


ABSTRACT: We established a whole tissue qXL-MS workflow to investigate proteome-wide changes in protein:protein interactions in mouse skeletal muscle insulin resistance (IR). Mice were feed a standard chow diet or a high fat diet (HFD) for 12 weeks which induced skeletal muscle insulin resistance. The Extensor digitorum longus (EDL) skeletal muscles were isolated and subjected to qXL-MS analysis. We chose this small fast-twitch muscle because it weighs ~8-10 mg providing sufficient protein yield (~1 mg) and it is commonly used in ex vivo myograph preparations due to adequate oxygen diffusion in O2/CO2-carbogen gassed Krebs-Henseleit bicarbonate-based buffer (Barclay, 2005). For XL-MS analysis of IR in skeletal muscle, chow and HFD EDL muscles (n=8 each) were incubated with 10 mM tPhoX for 2h.

INSTRUMENT(S):

ORGANISM(S): Mus Musculus (mouse)

TISSUE(S): Extensor Digitorum Longus

SUBMITTER: Benjamin Parker  

LAB HEAD: Benjamin Parker

PROVIDER: PXD077541 | Pride | 2026-05-29

REPOSITORIES: Pride

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