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Alterations in proteome and proteostasis networks during replicative senescence

ABSTRACT: The accumulation of senescent cells constitutes a pivotal hallmark of tissue ageing, contributing to disruption of tissue homeostasis and thus, to the development of several aging-related diseases. Despite this, our knowledge about the molecular, spatial and temporal changes that drive aging on a cellular basis remains poorly understood. To elucidate these mechanisms, we employed mass spectrometry-based proteomics to determine changes in the proteome and its post translational modifications, as well as protein solubility, during replicative senescence in human IMR90 fibroblasts. This accession contains the proteome data of proliferative and senescent cells, with a total of 200 000 cells were analyzed per condition.

INSTRUMENT(S):

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Fibroblast Of Lung

SUBMITTER: Petra Beli

LAB HEAD: Petra Beli

PROVIDER: PXD077797 | Pride | 2026-07-16

REPOSITORIES: Pride

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			Action	DRS
	2025-02-21-decoys-contam-HUMAN_release202301_UP000005640_9606_BSA_bovine.fasta.fas	Fasta
	20260401_AST1_PBE_NS_IMR90_same_cell_number_BSA_spike_in_Proliferative_R1.raw	Raw
	20260401_AST1_PBE_NS_IMR90_same_cell_number_BSA_spike_in_Proliferative_R1.raw.quant	Raw
	20260401_AST1_PBE_NS_IMR90_same_cell_number_BSA_spike_in_Proliferative_R2.raw	Raw
	20260401_AST1_PBE_NS_IMR90_same_cell_number_BSA_spike_in_Proliferative_R2.raw.quant	Raw

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Alterations in proteome and proteostasis networks during replicative senescence

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