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Effect of Magnesium ions on MEC performance and changes in proteome landscape in T. acetexigens

ABSTRACT: Microbial electrochemical systems (MECs) exploit the ability of electrogenic bacteria to transfer electrons to solid electrodes, thereby generating electricity or driving chemical synthesis. Model organisms such as Geobacter sulfurreducens and Shewanella oneidensis demonstrate how extracellular electron transfer (EET) can be harnessed in microbial fuel cells and electrolysers. While these systems show great promise, the performance of these devices depends on numerous factors, including pH, ionic strength and nutrient availability. Among those, magnesium has emerged as an especially important micronutrient because of its integral role in microbial physiology and its potential to influence electron transfer rates.This study examines how magnesium availability affects T. acetexigens performance. T. acetexigens is notable for its ability to produce current densities exceeding 9 A m-2 and possesses multiple co-existing EET pathways including large multi-heme cytochromes with up to 86 heme-binding motifs. Previously, we observed that the requirements for magnesium in growth media are significantly higher for T. acetexigens, compared to other EET bacteria, such as G. sulfurreducens. DSMZ (German Collection of Microorganisms) medium 148 for T. acetexigens specifies 1.6 mM MgSO4, whereas medium 826 for G. sulfurreducens contains only 0.12 mM. This represents a more than 13-fold difference in magnesium requirements. This observation prompted us to investigate how magnesium affects EET mechanisms, survivability and biofilm development along with associated changes on proteome level in T. acetexigens.

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ORGANISM(S): Desulfuromonas Acetexigens

SUBMITTER: Maksim Sysoev

LAB HEAD: Maksim Sysoev

PROVIDER: PXD078003 | Pride | 2026-05-13

REPOSITORIES: Pride

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Project description:Abstract Background Vertebral endplate signal intensity changes visualized by magnetic resonance imaging termed Modic changes (MC) are highly prevalent in low back pain patients. Interconvertibility between the three MC subtypes (MC1, MC2, MC3) suggests different pathological stages. Histologically, granulation tissue, fibrosis, and bone marrow edema are signs of inflammation in MC1 and MC2. However, different inflammatory infiltrates and amount of fatty marrow suggest distinct inflammatory processes in MC2. Aims The aims of this study were to investigate i) the degree of bony (BEP) and cartilage endplate (CEP) degeneration in MC2, ii) to identify inflammatory MC2 pathomechanisms, and iii) to show that these marrow changes correlate with severity of endplate degeneration. Methods Pairs of axial biopsies (n=58) spanning the entire vertebral body including both CEPs were collected from human cadaveric vertebrae with MC2. From one biopsy, the bone marrow directly adjacent to the CEP was analyzed with mass spectrometry. Differentially expressed proteins (DEPs) between MC2 were identified and bioinformatic enrichment analysis was performed. The other biopsy was processed for paraffin histology and BEP/CEP degenerations were scored. Endplate scores were correlated with DEPs. Results Endplates from MC2 were significantly more degenerated. Proteomic analysis revealed an activated complement system, increased expression of extracellular matrix proteins, angiogenic, and neurogenic factors in MC2 marrow. Endplate scores correlated with upregulated complement and neurogenic proteins. Discussion The inflammatory pathomechanisms in MC2 comprises activation of the complement system. Concurrent inflammation, fibrosis, angiogenesis, and neurogenesis indicate that MC2 is a chronic inflammation. Correlation of endplate damage with complement and neurogenic proteins suggest that complement system activation and neoinnervation may be linked to endplate damage. The endplate-near marrow is the pathomechanistic site, because MC2 occur at locations with more endplate degeneration. Conclusion MC2 are fibroinflammatory changes with complement system involvement which occur adjacent to damaged endplates.

			Action	DRS
	20250626_Maksim_HeLa_postrun_QC.raw	Raw
	Experimental_annotation.csv	Csv
	Maksim.predicted.speclib	Other
	Maksim.tsv	Tabular
	R1_low_final.raw	Raw

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Effect of Magnesium ions on MEC performance and changes in proteome landscape in T. acetexigens

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