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Proteomic analysis of 3T3 murine fibroblast used as feeder-layer

ABSTRACT: 3T3 murine embryonic fibroblasts are used as feeder layers to support the proliferative potential of human primary keratinocytes during in vitro expansion for cell and gene therapy applications. To identify signals mediating this function, we analyzed fibroblast protein content using mass spectrometry. We compared the proteome of lethally irradiated fibroblasts used as feeder layers in two conditions: standard quality (SQ-FL) and low quality (LQ-FL) fibroblasts. Our findings highlight differences in extracellular matrix components between the two conditions

INSTRUMENT(S):

ORGANISM(S): Mus Musculus (mouse)

TISSUE(S): Cell Culture, Fibroblast

SUBMITTER: Antonio Marino

LAB HEAD: Elena Enzo

PROVIDER: PXD078052 | Pride | 2026-05-18

REPOSITORIES: Pride

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			Action	DRS
	20241112_ASC1_NEO4_PRI_AUR3_25cm_60min_NO_FAIMS_X2242_P0339_R1444_S01.raw	Raw
	20241112_ASC1_NEO4_PRI_AUR3_25cm_60min_NO_FAIMS_X2242_P0339_R1444_S02.raw	Raw
	20241112_ASC1_NEO4_PRI_AUR3_25cm_60min_NO_FAIMS_X2242_P0339_R1444_S03.raw	Raw
	20241112_ASC1_NEO4_PRI_AUR3_25cm_60min_NO_FAIMS_X2242_P0339_R1444_S04.raw	Raw
	20241112_ASC1_NEO4_PRI_AUR3_25cm_60min_NO_FAIMS_X2242_P0339_R1444_S05.raw	Raw

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