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Mass Spectrometry Profiling of Lysosomal IP under Normoxia and Hypoxia-Reoxygenation

ABSTRACT: This project employed TMEM192-3×Flag-mediated lysosome immunoprecipitation (Lyso-IP) followed by mass spectrometry to investigate lysosomal proteomes under normoxia and hypoxia/reoxygenation (H/R) stress. Our study focuses on the mechanisms of lysosomal renewal, revealing that damaged lysosomes undergo budding-type fission (B-fission) to produce functional daughter lysosomes. The dataset provides a comprehensive resource for exploring stress-responsive lysosomal proteins and organelle quality control pathways.

INSTRUMENT(S):

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Cell Culture

SUBMITTER: Zhuofan Li

LAB HEAD: Zhiyin Song

PROVIDER: PXD078485 | Pride | 2026-06-03

REPOSITORIES: Pride

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			Action	DRS
	LYSO-IP-search.xlsx	Xlsx
	TMEM192-3xFlag-HpRe-1.raw	Raw
	TMEM192-3xFlag-HpRe-2.raw	Raw
	TMEM192-3xFlag-HpRe-3.raw	Raw
	TMEM192-3xFlag-N-1.raw	Raw

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