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Extracellular vesicles from Kluyveromyces marxianus as potential postbiotic against Candida albicans vaginal infections

ABSTRACT: A class of extracellular vesicles (EVs), isolated from the culture supernatant of a Kluyveromyces marxianus strain deriving from an artisanal sourdough, is reported. Previous work had clearly shown the probiotic properties of the yeast isolate, and its antagonistic activities against clinical fluconazole-resistant Candida albicans strains. Characterization of the isolated EVs by nanotracking particle analysis showed they had a mean diameter of 157,7 nm. Proteomic characterization of the purified EVs identified a complex array of 100 proteins. Both C. albicans planktonic growth and biofilm formation were inhibited by the isolated EVs, as well as adhesion and invasion of Candida cells in the vaginal epithelial A-431 cells. In the same cell model, EVs from K. marxianus exerted an immunomodulatory effect affecting the secretion of pro-inflammatory and anti- inflammatory cytokines. Also, the expression of Candida SAP2 and SAP6 genes, coding two aspartyl proteases involved in the invasion and damage of the epithelial mucosa, was affected by the presence of EVs. On the whole, the results showed that EVs from the probiotic K. marxianus retain, at least in part, the beneficial features of the live microrganism, so representing a postbiotic cell-free alternative preparation potentially useful for the management of C. albicans vaginal infections.

INSTRUMENT(S):

ORGANISM(S): Ascomycota Kluyveromyces Marxianus

SUBMITTER: CHIARA D'AMBROSIO

LAB HEAD: Chiara D'Ambrosio

PROVIDER: PXD078607 | Pride | 2026-06-25

REPOSITORIES: Pride

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	KM1_10.raw	Raw
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