Relaxin-2: shaping the proteomic landscape of skeletal muscle physiology, glucose trafficking, and mitochondrial function in rat.

ABSTRACT: Relaxin-2 is a hormone with robust beneficial effects on the heart and blood vessels and with a potential therapeutic efficacy in cardiovascular (CV) disease. Considering the interorgan communication between skeletal muscle and heart, and the relation between muscle quality/composition and CV events, we hypothesise that relaxin-2 may regulate skeletal muscle physiology and metabolism. We aim to evaluate the impact of serelaxin on the proteome of skeletal muscle from healthy Sprague-Dawley rats. Animals were treated with 0.4 mg/kg/day of serelaxin (recombinant form of human relaxin-2) or vehicle (PBS) for 14 days employing subcutaneous osmotic minipumps. Skeletal muscle proteomic profiles were analysed using SWATH/MS quantification and qualitative data-dependent workflow analysis (DDA) carried out in a TripleTOF 6600 System. Quantitative analysis identified that serelaxin significantly decreased 89 proteins and significantly increased 31 proteins in rat skeletal muscle when compared to control rats. From these, 34 proteins are associated with muscle function, myogenesis, muscle differentiation and/or regeneration, 15 are mitochondrial proteins (6 of them subunits of the complexes of the electron transport chain), and 10 proteins participate in glucose metabolism. Qualitative analysis identified 35 proteins exclusive to the skeletal muscle of the serelaxin-treated group: 8 proteins related to processes of muscle function (regulation of skeletal muscle size, ion homeostasis or organisation of caveolae structures and cytoskeleton) and myogenesis, and 2 proteins involved in muscle differentiation. We highlight the role of serelaxin shaping the skeletal muscle proteome, which could influence several processes involved in myopathy and CV, such as mitochondrial dysfunction and bioenergetic abnormalities.

INSTRUMENT(S):

ORGANISM(S): Rattus Norvegicus (rat)

TISSUE(S): Skeletal Muscle

SUBMITTER: Susana Bravo  

LAB HEAD: Susana B Bravo

PROVIDER: PXD078619 | Pride | 2026-09-21

REPOSITORIES: Pride