Identification of Plk1-PBD interacting proteins

ABSTRACT: This project aims to identify Plk1-PBD interactors potentially involved in DNA repair. We performed unbiased proteomic screens in S and G2 phases. HeLa cells were synchronized by double-thymidine block-and-release, and pull-downs were conducted with GST-Plk1 PBD as bait. GST alone and a phospho-binding-deficient GST-PBD mutant (H538A/K540M) served as negative controls. Proteins were then analyzed by mass spectrometry.

INSTRUMENT(S):

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Epithelial Cell, Hela Cell

DISEASE(S): Cervix Carcinoma

SUBMITTER: Shinan Zhou  

LAB HEAD: Fangwei Wang

PROVIDER: PXD078902 | Pride | 2026-08-01

REPOSITORIES: Pride