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Mice BMDCs LCMS datta analysis for showing cross presentation in Antigen presenting cells

ABSTRACT: We have used mature BMDCs stimuated with flu peptide pool and did immunoprecipitation with MHC I antibody and followed acid elution to purified the CD8 T cell binding epitope.

INSTRUMENT(S):

ORGANISM(S): Mus Musculus (mouse)

TISSUE(S): Dendritic Cell, Bone Marrow

SUBMITTER: Rajesh Yadav

LAB HEAD: Rajesh Yadav

PROVIDER: PXD079132 | Pride | 2026-06-02

REPOSITORIES: Pride

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			Action	DRS
	3rd_repeat_MHCI_no_pep_antigen_alone.mzid	Mzid
	3rd_repeat_MHCI_pep_antigen_alone.mzid	Mzid
	MHC1_No_Pep.mzML	Mzml
	MHC1_Pep.mzML	Mzml
	MHC1_Pep.psdb	Other

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Mice BMDCs LCMS datta analysis for showing cross presentation in Antigen presenting cells

Project description:We have used mature BMDCs stimuated with flu peptide pool and did immunoprecipitation with MHC I antibody and followed acid elution to purified the CD8 T cell binding epitope.

2026-05-28 | PXD078927 | Pride

Effect of infection with Aggregatibacter actinomycetemcomitans or Porphyromonas gingivalis on primary murine bone marrow-derived dendritic cells

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Gene expression profiling in wild-type, Irf5-KO, Lyn-KO and Lyn/Irf5-DKO BMDCs unstimulated or stimulated with CpG-B ODN

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Project description:genes regualted by LPS or LPS+cAMP stimulation in BMDCs; We used microarrays to identify genes that up-regulated by LPS+cAMP compared with just LPS. Experiment Overall Design: BMDCs were stimilated with LPS (10 ng/ml) in the presence or absence of cAMP (100 microM) for 3h. Specifically up-regualted gene by cMP was identified.

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Transcription profiling of mouse bone marrow-derived dendritic cells (BMDC), with cells that had been cocultured (1:5, tumor:BMDC) with mouse ovarian surface epithelial cell line (1D8) cells, with irradiated (50Gy) 1D8 cells, or with the supernatent from 1D8 cells (25%, v/v)

Project description:The Suppressor of cytokine signaling (SOCS) family of negative regulatory proteins are upregulated in response to several cytokines and pathogen-associated molecular patterns (PAMPs), and suppress cellular signaling responses by binding receptor phosphotyrosine residues. Exposure of bone marrow-derived dendritic cells (BMDCs) to 1D8 cells, a murine model of ovarian carcinoma, suppresses their ability to express CD40 and stimulate antigen specific responses in response to PAMPs, and in particular to poly I: C with the upregulated SOCS3 transcript and protein levels. The ectopic expression of SOCS3 in both the macrophage cell line RAW264.7 and BMDCs decreased signaling in response to both poly I:C and IFNÎ±. Further, knockdown of SOCS3 transcripts significantly enhanced the responses of RAW264.7 and BMDCs to both poly I: C and IFNÎ±. Immunoprecipitation and pull-down studies demonstrate that SOCS3 binds to the IFNÎ± receptor TYK2. Since poly I: C triggers autocrine IFNÎ± signaling, binding of SOCS3 to TYK2 may thereby suppress the activation of BMDCs by polyI:C and IFNÎ±. Thus, elevated levels of SOCS3 in tumor-associated DCs may potentially resist the signals induced by TLR3 ligands and type I interferon to decrease DC activation via binding with IFNÎ± receptor TyK2. Experiment Overall Design: Microarray analysis was used to compare the expression levels of Control mouse bone marrow-derived dendritic cells (BMDC), with cells that had been cocultured (1:5, tumor:BMDC) with mouse ovarian surface epithelial cell line (1D8) cells, with irradiated (50Gy) 1D8 cells, or with the supernatent from 1D8 cells (25%, v/v). Experiment Overall Design: One biologic sample was analyzed for each condition, four samples in all.

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Control of RelB during dendritic cell activation integrates canonical and non-canonical NFkB pathways

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