Micro- and macro- heterogeneity of N-glycoproteome remodeling in endoplasmic reticulum stress

ABSTRACT: We developed an optimized magnetic polyethyleneimine boronic acid-containing scaffold (mPBA) enrichment workflow to achieve sensitive and broad enrichment of intact glycoproteins for mass spectrometry analysis, quantifying 13759 unique protein-, site-, and glycoform combinations, termed glycopeptidoforms, in normal (AC16, HeLa, hiPSC-CM) and stressed (AC16, hiPSC-CM) human cells while requiring only 0.1 to 0.5 mg total peptide input.

INSTRUMENT(S):

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Cell Culture

SUBMITTER: Maggie Pui Yu Lam  

LAB HEAD: Margaret (Maggie) Lam

PROVIDER: PXD079324 | Pride | 2026-10-19

REPOSITORIES: Pride