Dataset Information

SpxA1 and SpxA2 Function as a Stoichiometry-Dependent Regulatory Rheostat Governing Virulence Gene Expression in Group A Streptococcus — DIA Proteomics

ABSTRACT: Group A Streptococcus (GAS) encodes two paralogs of the Spx transcriptional regulator, SpxA1 and SpxA2, which interact with RNA polymerase to modulate virulence gene expression. SpxA2 is directly regulated by the LiaFSR three-component system in response to antimicrobial peptide stress. To define how SpxA2 influences CovR DNA occupancy genome-wide, we performed chromatin immunoprecipitation with exonuclease treatment (ChIP-exo) targeting CovR in wild-type MGAS10870 (emm3), an isogenic spxA2 deletion mutant (deltaSpxA2), and a LiaSQ146A constitutively active LiaS mutant used as a SpxA2 overexpression surrogate (SpxA2-OE). Differential binding analysis identified 439 sites with significantly altered CovR occupancy between wild-type and deltaSpxA2 (FDR < 0.05), with class-specific enrichment of CovR dimer and monomer binding motifs consistent with the two-class CovR binding model (Horstmann et al. 2023). These data contribute to a multi-omic analysis including RNA-seq, DIA proteomics, and NanoString nCounter that defines the regulatory roles of SpxA1 and SpxA2 in GAS pathogenesis.

INSTRUMENT(S):

ORGANISM(S): Streptococcus Pyogenes Mgas315

SUBMITTER: Anthony Flores

LAB HEAD: Anthony R. Flores

PROVIDER: PXD079489 | Pride | 2026-07-13

REPOSITORIES: pride

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Dataset's files

Source:

			Action	DRS
	HT_6775_20260131_DIA30_2ul_A1KO-rep1_Slot1-19_1_8117.d.zip	Other
	HT_6775_20260131_DIA30_2ul_A1KO-rep2_Slot1-20_1_8120.d.zip	Other
	HT_6775_20260131_DIA30_2ul_A1KO-rep3_Slot1-21_1_8123.d.zip	Other
	HT_6775_20260131_DIA30_2ul_A1OE-rep1_Slot1-22_1_8126.d.zip	Other
	HT_6775_20260131_DIA30_2ul_A1OE-rep2_Slot1-23_1_8129.d.zip	Other

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Publications

SpxA1 and SpxA2 Function as a Stoichiometry-Dependent Regulatory Rheostat Governing Virulence Gene Expression in Group A Streptococcus.

Morrison Gretchen A GA Vega Luis Alberto LA Iglesias Misú Sanson MS Holly Khufu K McDonald W Hayes WH Horstmann Nicola N Shelburne Samuel A SA Gaddy Jennifer A JA Flores Anthony R AR

bioRxiv : the preprint server for biology 20260619

Group A Streptococcus (GAS) is a human-restricted pathogen whose global incidence has surged in the post-COVID era. The ability of GAS to shift from a colonizing to invasive phenotype depends on coordinated virulence gene regulation in response to host-derived signals. However, the mechanisms by which individual stress-sensing systems interact to reshape the virulence gene regulatory landscape remain incompletely understood. Here we define the regulatory programs of two conserved transcriptional ...[more]

PMID: 42367948

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