Systemic Proteome Remodeling in Soybean During Nodulation (responses in roots)

ABSTRACT: The aim of this project was to study rhizobia-induced proteome changes in soybean roots during early nodulation, with potential links with the autoregulation of nodulation pathway. Gene expression analysis of marker genes of the pathway, namely RIC1, RIC2, miR2111 and TML1a/1b, allowed to select three relevant time points for proteome analysis: 0, 3 and 7 dpi. Of these three, only 0 and 3 dpi were later used for downstream analysis. Soybean plants, cultivar Wm82, were grown in aeroponics for two weeks and inoculated with Bradyrhizobium diazoefficiens bjGFP. Roots were harvested at the selected time points, excluding root tips. Three technical replicates were included. Proteome dynamics in the leaves were also analyzed and are uploaded in PRIDE as well.

INSTRUMENT(S):

ORGANISM(S): Glycine Max

TISSUE(S): Root

SUBMITTER: Afroditi Katsaouni  

LAB HEAD: Sofie Goormachtig; Kris Gevaert

PROVIDER: PXD079829 | Pride | 2026-10-19

REPOSITORIES: Pride