Dataset Information

An integrated proteome and phospho-proteome approach to study BPA toxicity on placenta.

ABSTRACT: The environmental exposure of various Endocrine disrupting chemicals (EDCs) like Bisphenol-A (BPA), can affect human health by inducing hormonal imbalance important for physiological homeostasis. Pregnant females are more susceptible for BPA mediated toxicity. BPA can reach maternal circulation by ingestion, inhalation or dermal contact from plastic goods or everyday house-hold items, which then crosses the placental barrier and imparts damage on trophoblast cells. This BPA mediated cellular injury directly alters feto-maternal homeostasis disrupting fetal development and growth. The feto-maternal disruption is induced by impaired cellular signaling in placenta, which can also affect the post translational protein phosphorylation patterns. Therefore, this study explores the dynamic proteome change upon BPA exposure which also affects the kinase associated phospho-signatures in both early trimester trophoblast cells and murine placenta. This finding provides an important insight on the mechanism of altered phosphorylation which may drive the BPA associated toxicity in placenta and trophoblast cells contributing towards placenta mediated reproductive disorders.

INSTRUMENT(S):

ORGANISM(S): Homo Sapiens (human) Mus Musculus (mouse)

TISSUE(S): Extravillous Trophoblast, Placenta, Cell Culture

SUBMITTER: TUSHAR KANTI MAITI

LAB HEAD: Tushar Kanti Maiti

PROVIDER: PXD080006 | Pride | 2026-07-14

REPOSITORIES: Pride

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			Action	DRS
	HTR8_BPA_PhosVal_HRMRM_UnSH_BPA1_R1A_29052026.timeseries.data	Other
	HTR8_BPA_PhosVal_HRMRM_UnSH_BPA1_R1A_29052026.wiff	Wiff
	HTR8_BPA_PhosVal_HRMRM_UnSH_BPA1_R1A_29052026.wiff.scan	Wiff
	HTR8_BPA_PhosVal_HRMRM_UnSH_BPA1_R1A_29052026.wiff2	Wiff
	HTR8_BPA_PhosVal_HRMRM_UnSH_BPA1_R1B_29052026.timeseries.data	Other

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Project description:We used the rhesus monkey (Macaca mulatta) as our animal model for the current study with two goals: to characterize the changes in histology and gene expression from early to late gestation (prenatal uterine organogenesis) and to determine if there are effects of prenatal exposure to bisphenol A (BPA) on the developing female uterus. Pregnant rhesus monkeys carrying a female fetus (N=22) were divided into two experimental groups, based on gestational timing: 'early' (N=10) and 'late' (N=12). These groups were then equally sub-divided into control (unexposed) and BPA (exposed) groups (5 Early Control, 5 Early BPA-exposed, 6 Late Control and 6 Late BPA-exposed.) The BPA-exposed monkeys received a deuterated BPA (dBPA, CDN Isotopes, Quebec, Canada) fruit treat on a daily basis, at a dose of 400ug/kg/day). The dosing was aimed at achieving serum levels of BPA detected in adult human biomonitoring studies. The control animals received a vehicle control on a daily basis. The 'early' time period (mid-gestation) referred to gestational days 50-100, approximating the second trimester of human gestation. The fetal monkeys in the 'early' group were delivered via cesarean section on gestation day 100 and euthanized. Samples of maternal and fetal blood and amniotic fluid were obtained. Maternal and fetal weights were also recorded. The 'late' time period referred to gestational days 100-165, approximating the third trimester of human gestation. The fetal monkeys in the 'late' group were delivered vaginally and euthanized. There were five idiopathic stillbirths (2 Control, 3 BPA-exposed) in the late group. Samples of maternal and fetal blood and amniotic fluid were obtained. Maternal and fetal weights were also recorded. After delivery, the fetal uterus was excised and cut sagitally from fundus to cervix; one side was fixed for histological evaluation and the other half was frozen for analysis of gene expression by microarray. The stillbirths were excluded from the microarray.

Project description:Compairsion of transcriptional profiles of heart and skeletal muscle tissue of fetal rhesus monkey exposed to maternal Bisphenol A or vehicle during early or late gestaion. Maternal exposure to the endocrine disrupting chemical, bisphenol A (BPA) affects the development of multiple organ systems in rodents and monkeys. However, effects of BPA exposure on cardiac and skeletal muscle development have not been assessed. Given that maternal BPA crosses placenta and reaches developing fetus, examining the physiological consequences of gestational exposure during development is of research significance. Therefore, we evaluate the effects of daily, oral BPA exposure of pregnant rhesus monkeys (Macaca mulatta) on the fetal heart and skeletal muscle transcriptome. Pregnant monkeys were administered daily oral doses (400 M-BM-5g/kg body weight) of BPA during early (50 M-bM-^@M-^S100 M-BM-1 2 days post conception, dpc) or late (100 M-BM-1 2 dpc - term), gestation. At the end of treatment, fetal heart tissues; left ventricle (LV), right ventricle (RV), left atrium (LA), right atrium (RA) and skeletal muscle; biceps femoris (BFM), were collected. Transcriptome expression was assessed using genome-wide microarray in each of the tissues and compared paired-wise between the BPA and matched control fetuses. Our results show that maternal BPA exposure alters transcriptional profile of several coding and non-coding genes in fetal heart and skeletal muscle. Pregnant rhesus monkey were administered a daily oral dose of 400 M-NM-<g/kg. body weight of Bisphenol A (BPA) or vehicle (CON) either during early (50M-bM-^@M-^S100 M-BM-1 2 days) or late (100 M-BM-1 2 daysM-bM-^@M-^Sterm) gestation. Gene expression profiles of each of the heart chambers (left ventricle, LV; right ventricle, RV; left atrium, LA; and right atrium, RA) and skeletal muscle (biceps femoris, BFM) were analyzed using microarrays and compared between the BPA exposed and matched control fetuses. A total of 12 samples were analyzed for each tissue; LV, RV, LA, RA and BFM. This includes 6 samples at each time period (early vs. late gestation) and 3 biological replicates for each treatment (BPA, n=3; control, n=3).

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An integrated proteome and phospho-proteome approach to study BPA toxicity on placenta.

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