Identifying the mitotic interactome of the human PLK1 protein kinase

ABSTRACT: The project mapped the interactors of PLK1 in cells were the endogenous PLK1 locus was tagged with 3xHA tags. Cells were arrested in mitosis with nocodazole and treated with a PLK1 inhibitor (BI-2536) to trap PLK1 onto its substrates, facilitating detection by mass spectrometry.

INSTRUMENT(S):

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Cell Culture

SUBMITTER: Renata Soares  

LAB HEAD: John Rouse

PROVIDER: PXD080479 | Pride | 2026-07-03

REPOSITORIES: Pride