Proteomics

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Human gastric cardia adenocarcinoma LC-MSMS


ABSTRACT: Therapeutic targets for gastric cardia adenocarcinoma (GCA), particularly for HER2-negative patients, are lacking. Here, we conducted multi-omics profiling on 128 GCA patients using Mass spectrometry, whole-exome sequencing, RNA-Seq, scRNA-Seq and spatial transcriptomics. Based on HER2 expression levels, we categorized patients into HER2-high, - low, and -negative groups and confirmed the favorable prognostic significance of HER2. We uncovered an enrichment of DNA repair features in the HER2-high group, while HER2-low and -negative groups exhibited strong inflammation. We found that tumor mutation burden may not be the distinguishing factor among these three groups. We revealed that the HER2- negative and -low groups have a tumor-suppressive immune microenvironment. Our study revealed that anti-inflammatory approaches and immune checkpoint inhibition targeting the CD47/SIRPA axis may serve as therapeutic strategies for patients with HER2-negative GCA. These findings highlight promising avenues for personalized treatment of GCA.

INSTRUMENT(S):

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Gastroesophageal Junction

SUBMITTER: Li Dong Wang  

LAB HEAD: Li Dong Wang

PROVIDER: PXD080883 | Pride | 2026-07-12

REPOSITORIES: Pride

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