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Succinate and its carrier Sfc1 mediate metabolic control of mitochondrial protein import by the TIM23 translocase

ABSTRACT: Tim23 is an essential component of the mitochondrial inner membrane translocase and Sfc1 is a carrier that exchanges succinate for fumarate across that membrane. Sfc1 and succinic acid availability regulate dual targeting of fumarase and aconitase by facilitating mitochondrial import of their newly synthesized precursors, as shown by pulse-chase experiments. Here we show that Sfc1 associates with Tim23 in vivo, and succinate modulates this association, which in turn affects mitochondrial protein import. Physical interaction between Tim23 and Sfc1 was proven by co-immunoprecipitation, Bimolecular Fluorescence Complementation (BiFC) and Biotin-based proximity labeling (TurboID). Proximity labeling and structural modelling-informed mutagenesis allowed us to dissect the carrier activity of Sfc1 from its function as a TIM23 regulator. We performed Rosetta-MP docking of Sfc1 and Tim23 to envisage the interface. Thus, our findings show that metabolites can regulate mitochondrial import and adjust the segregation of key metabolic enzymes between the cytosol and mitochondria.

INSTRUMENT(S):

ORGANISM(S): Saccharomyces Cerevisiae (baker's Yeast)

SUBMITTER: Tamar Ziv

LAB HEAD: Ophry Pines

PROVIDER: PXD081371 | Pride | 2026-07-21

REPOSITORIES: Pride

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			Action	DRS
	Seq71282_QE2.msf	Msf
	Seq71282_QE2.raw	Raw
	Seq71283_QE2.msf	Msf
	Seq71283_QE2.raw	Raw
	Seq71284_QE2.msf	Msf

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