Legionella effector RavH is a tandem WH2-like domain-containing PI(3)P-dependent actin nucleator

ABSTRACT: To decipher the mechanisms by which RavH modulates F-actin structures, we performed an affinity purification based SILAC (Stable Isotope Labeling with Amino Acids in Cell Culture) coupled mass spectrometry (MS)toidentify RavH-associated proteins. Cells expressing GFP-RavH or GFP alone were subjected to GFP immunoprecipitation, followed by quantitative MS analysis. We found that beta-actin was highly enriched when GFP-RavH was used as bait.

INSTRUMENT(S):

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Permanent Cell Line Cell, Cell Culture

SUBMITTER: Mateusz Wagner  

LAB HEAD: Yuxin Mao

PROVIDER: PXD082146 | Pride | 2026-08-14

REPOSITORIES: Pride