Development of a laser injury model using human cardiac spheroids

ABSTRACT: This study establishes a laser injury model using 3D cardiac spheroids composed of human induced pluripotent stem cell-derived cardiomyocytes (iPSC-CMs) and cardiac fibroblasts (iPSC-CFs). Following the in vitro differentiation of both iPSC-CMs and iPSC-CFs, the two cell types are co-cultured to allow self-aggregation into cardiac spheroids. Focal injury is induced via targeted laser ablation, after which the injured spheroids are cultured for 7 days post-ablation to observe biological responses. Spheroids are subsequently harvested and snap-frozen in liquid nitrogen for downstream molecular and histological analysis.

INSTRUMENT(S):

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Cell Culture, Cardiofibroblast

SUBMITTER: Manfred Heller  

LAB HEAD: Prof. Dr. Nadia Mercader Huber

PROVIDER: PXD082285 | Pride | 2026-10-16

REPOSITORIES: Pride