Phosphorylation Patterns and Digestion-Derived Peptide Profiles of Precision-Fermented αS1-Casein

ABSTRACT: This study characterizes phosphorylation patterns in precision-fermented αS1-casein produced recombinantly in Escherichia coli, comprising a monomeric construct (CF AS1) and a tandem-fusion construct (CF AS1-AS1) engineered to duplicate the native αS1-casein domain, benchmarked against a commercial bovine αS1-casein reference standard (SIG AS1). Bottom-up phosphoproteomic LC-MS/MS analysis was performed to identify canonical and non-canonical phosphorylation sites across all three constructs, with phosphosite localization determined using MaxQuant. This dataset comprises the bottom-up phosphoproteomic raw data and corresponding MaxQuant search results supporting the findings reported in the associated manuscript.

INSTRUMENT(S):

ORGANISM(S): Bos Taurus (bovine)

SUBMITTER: Yasuri Balasooriya Mudiyanselage  

LAB HEAD: Balasooriya Mudiyanselage Yasuri Dinushka Ekmini Amarasekara

PROVIDER: PXD082728 | Pride | 2026-10-15

REPOSITORIES: Pride