An improved capillary isoelectric focusing-mass spectrometry method for characterization of monoclonal antibody charge variants

ABSTRACT: Here, we improved the stability and enhanced separation resolution of automated cIEF-MS by bettering the quality of capillary neutral coating, reducing catholyte pH to 10 for cIEF-MS for the first time, and systematically optimizing the cIEF separation conditions for characterization of monoclonal antibody charge variants.

INSTRUMENT(S):

ORGANISM(S): Homo Sapiens (human)

SUBMITTER: Liangliang Sun  

LAB HEAD: Liangliang Sun

PROVIDER: PXD082799 | Pride | 2026-08-19

REPOSITORIES: Pride