Proteomics

Dataset Information

Mass spectrometry characterization of O-GlcNAc modification of GFP-tagged G3BP1

ABSTRACT: This dataset contains mass spectrometry data generated to characterize O-GlcNAc modification of human G3BP1 expressed as an N-terminal EGFP fusion in H9c2 cardiac cells. EGFP-G3BP1 was overexpressed by adenoviral transduction, affinity purified using GFP-Trap, resolved by SDS-PAGE, and the band corresponding to EGFP-G3BP1 was excised for mass spectrometric analysis. HCD-triggered EThcD acquisition was used to identify HexNAc-modified peptides and localize modification sites. The dataset provides peptide sequence coverage and site-level information for G3BP1 O-GlcNAcylation, including localization of a HexNAc modification to T268 as reported in the associated study.

INSTRUMENT(S):

ORGANISM(S): Rattus Norvegicus (rat) Homo Sapiens (human)

TISSUE(S): Cardiocyte

SUBMITTER: Kyriakos Papanicolaou  

LAB HEAD: Kyriakos N Papanicolaou

PROVIDER: PXD083651 | Pride | 2026-09-28

REPOSITORIES: Pride

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Dataset's files

Source:
Action DRS
221006_PapanicolaouK_KP_Scaffold.sf3 Other
RO-CS-LE_221006_PapanicolaouK_KP_1a-b.msf Msf
RO-CS-LE_221006_PapanicolaouK_KP_1a-b.mzML Mzml
RO-CS-LE_221006_PapanicolaouK_KP_1a-b.pdResult Other
RO-CS-LE_221006_PapanicolaouK_KP_1a-b.raw Raw
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