Three-dimensional spheroid and bioprinted cervical cancer models exhibit non-interchangeable proteomic states

ABSTRACT: This dataset supports a comparative proteomic study of HeLa and SiHa cervical cancer cells cultured as adherent monolayers, scaffold-free spheroids, and 3D bioprinted alginate/gelatin constructs. The study integrated morphological assessment, resazurin-based relative metabolic activity measurements, and DIA-PASEF label-free quantitative proteomics to evaluate how culture architecture affects cervical cancer cell phenotypes. The global proteomic dataset comprised 8,837 protein groups and showed architecture-dependent organization in both cell lines. Spheroid models were preferentially associated with glycolytic remodeling, metabolic adaptation, and hypoxia-associated signatures, whereas bioprinted models showed stronger representation of extracellular matrix remodeling, cell–matrix interaction, and selected coagulation/complement-associated protein remodeling. Direct comparison between spheroid and bioprinted models confirmed that these 3D architectures generated distinct proteomic states rather than interchangeable alternatives to two-dimensional culture.

INSTRUMENT(S):

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Permanent Cell Line Cell, Cell Culture

DISEASE(S): Cervix Carcinoma

SUBMITTER: Pablo Saldivia  

LAB HEAD: Mauricio Hernández

PROVIDER: PXD084972 | Pride | 2026-10-23

REPOSITORIES: Pride