Systirin (CCDC201) immunoprecipitation from differentiated human induced pluripotent stem cells: wild type versus Systirin knockout

ABSTRACT: Sister chromatid cohesion must be established and then maintained across the extended prophase of gametogenesis. In mitosis this depends on Sororin, but no equivalent factor had been identified for meiotic cohesin. We identify Systirin, the protein encoded by CCDC201, as a conserved, germ cell-restricted paralog of Sororin that binds REC8-cohesin and is required for meiotic chromosome axis formation and sister chromatid cohesion. This dataset contains the immunoprecipitation-mass spectrometry experiment used to confirm loss of Systirin protein in CRISPR-Cas9 Systirin knockout clones. Endogenous Systirin was immunoprecipitated with a custom sheep anti-Systirin antibody from male PGP1 human induced pluripotent stem cells (DDX4-T2A-tdTomato/REC8-T2A-mGreenLantern reporter carrying integrated EF1a-BCL2 and doxycycline-inducible BOLL, HOXB5 and MEIOC) differentiated towards a meiosis I-like state, in the parental wild-type background and in two independent Systirin knockout clones. Spectral counts and protein intensities were used to assess the level of Systirin in wild-type versus knockout cells. The dataset accompanies Tsukada et al., “Systirin mediates meiotic sister chromatid cohesion”, Nature Structural & Molecular Biology (2026), where it is reported as Supplementary Table 1.

INSTRUMENT(S):

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Pluripotent Stem Cell

SUBMITTER: Andrew Blackford  

LAB HEAD: Andrew Blackford

PROVIDER: PXD085158 | Pride | 2026-10-21

REPOSITORIES: Pride