Project description:Neurospora intermedia is a heterothallic filamentous ascomycete. In this study we use microarray technology to study the difference in gene expression between vegetative growth and early reproductive development. Neurospora intermedia FGSC#8882 mat-A and FGSC#8782 mat-a. Solid synthetic crossing medium (SCM) was used as a nutrient regime before sampling and processing. Two different conditions were sampled: vegetative mycelial tissue and young reproductive mycelial tissue.
Project description:Neurospora intermedia is a heterothallic filamentous ascomycete. In this study we use microarray technology to study the difference in gene expression between vegetative growth and early reproductive development.
Project description:Transcriptional profiling with next-generation sequencing methods demonstrated that a Neurospora crassa mutant with the three most highly expressed beta-glucosidase genes deleted had a transcriptional response to cellobiose similair to that of wild type N. crassa exposed to cellulose.
Project description:The goals of this study are to compare Next-Generation-Sequecing (NGS)-derived genome-wide occupancy of H3 and H4 acetylation in S. cerevisiae wildtype and gds1 deletion strain.
Project description:In this study, we found that ADAR1 reduces the levels of double-stranded RNAs (dsRNAs) derived from both nuclear and mitochondrial transcripts. To elucidate how ADAR1 controls mitochondrial dsRNA (mt-dsRNA) accumulation, we performed quantitative mass spectrometry on mitochondrial protein fractions from wild-type and ADAR1-deficient cells, aiming to identify mitochondrial protein mediators involved in ADAR1-dependent dsRNA regulation.
Project description:We previously reported that colorectal cancer sphere cells express CD44v6, which is required for their migration and generation of metastatic tumors. The goal of this study is to compare NGS-derived transcriptome profiling (RNA-seq) obtained from enriched CD44v6+ and CD44v6- fractions from sphere cells harboring different mutations.
Project description:Loss of SAMHD1 causes chronic activaiton of the MDA5/MAVS dsRNA sensing pathway, only when cGAS/STING signaling is intact. Peritoneal macrophages from mutant and control mice were isolated by FACS. Total RNA was subjected to next generation mRNA sequencing.
Project description:Loss of SAMHD1 causes chronic activaiton of the MDA5/MAVS dsRNA sensing pathway, only when cGAS/STING signaling is intact. Peritoneal macrophages from mutant and control mice were isolated by FACS. Total RNA was subjected to next generation mRNA sequencing.