Project description:Moorella thermoacetica-CdS hybrid system have been proven capable of efficiently harnessing solar energy into chemical energy. This project aims to make clear the concrete electron transport pathway, CO2 fixation process and energy production for this photosynthetic biobybrid system with the help of quanlitative and label free quantitative proteomics.
Project description:Moorella thermoacetica spores are the most heat-resistant so far retrieved in food industry and we previously showed that the resistance properties of these spores to wet- heat and biocides were lower when spores were produced at low limit temperature than at optimal temperature. By electron microcopy, we observed that the ultrastructure of the spore coat differed according to the sporulation temperature, with spores produced at 55 °C mainly exhibiting lamellar inner coat tightly associated to diffuse outer coat, while spores produced at 45 °C showing an inner and outer coat separated by a less electron- dense zone. Moreover, misarranged coat structures were more frequently observed when spores were produced at low limit temperature. We analyzed the proteome of spore ob- tained at 45° and 55 °C and focused our data analysis on putative spore coat, exosporium proteins or proteins playing a role in spore resistance. Some putative spore coat proteins, such as CotSA, were only identified in spores produced at 55 °C, while some other puta- tive exosporium and coat proteins were significantly less abundant in spores produced at 45 °C. Altogether, our results suggest that sporulation temperature affects the structure and the protein composition of M. thermoacetica spores.
Project description:Proteomic analysis was utilized to explore the effects on the microorganism induced by the carbon-based semiconductor. We then characterized the proteomic and changes of the proposed M. thermoacetica-g-C3N4 and Moorella thermoacetica-CdS hybrid system during light harvesting, and proposed the electron transfer, energy generation and CO2 fixation mechanism.
