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Scleractinia

ABSTRACT: Transcriptome analysis of egg and embryonic samples of various corals

PROVIDER: PRJDB11460 | ENA |

REPOSITORIES: ENA

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Dataset's files

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			Action	DRS
	DRR286406_1.fastq.gz	Fastqsanger.gz
	DRR286406_2.fastq.gz	Fastqsanger.gz
	DRR286407_1.fastq.gz	Fastqsanger.gz
	DRR286407_2.fastq.gz	Fastqsanger.gz
	DRR286408_1.fastq.gz	Fastqsanger.gz

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Comparative Evaluation of Five Protocols for Protein Extraction from Stony Corals (Scleractinia) for Proteomics

Project description:Corals especially the reef-building species are very important to marine ecosystems. Proteomics has been used for researches on coral diseases, bleaching and responses to the environment change. Corals especially the reef-building species are very important to marine ecosystems. Proteomics has been used for researches on coral diseases, bleaching and responses to the environment change. In the present study, five protocols were compared for protein extraction from stony corals.

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MicroRNA expression profiles from eggs of different qualities associated with post-ovulatory ageing in rainbow trout

Project description:Egg quality is an important aspect in rainbow trout farming. Post-ovulatory aging is one of the most important factors affecting egg quality. MicroRNAs (miRNAs) are the major regulators in various biological processes and their expression profiles could serve as reliable biomarkers for various pathological and physiological conditions. Egg samples from 32 females on day 1, day 7, and day 14 post-ovulation (D1PO, D7PO and D14PO), which had the fertilization rates of 91.8%, 73.4% and less than 50%, respectively, were collected and small RNAs isolated from these samples were subjected to deep sequencing using the Illumina platform. Six miRNAs were found to be differentially expressed between D1PO and D14PO eggs. GO analysis of the target genes of the 6 miRNAs that were down-regulated in D14PO eggs revealed significantly enriched GO terms that are related to stress response, cell death, DNA damage, ATP generation, signal transduction and transcription regulation.

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Bacterial Isolates from Scleractinia Coral

Project description:This data set contains bacteria isolated from corals. The bacteria were cultured and tested in a biological assay against pathogenic bacteria that may be involved in Stony Coral Tissue Loss Disease. The bacteria in this data set include pathogenic and potentially probiotic bacteria.

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Variation in embryonic mortality and maternal transcript expression among Atlantic cod (Gadus morhua) females: a functional genomics study

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Project description:The present study aimed at studying the rainbow trout egg transcriptome using 9152-cDNA microarrays after natural or controlled ovulation. The analysis of egg transcriptome after natural or controlled ovulation led to the identification of 26 genes. We observed that both hormonal induction and photoperiod control of ovulation induced significant changes in the egg mRNA abundance of specific genes. We demonstrate that hormonal induction of ovulation has an impact on the egg mRNA abundance of specific genes even though the resulting effects on the developmental potential of the egg is so far unknown. In addition, we also identified 1 gene exhibiting a differential mRNA abundance in eggs of varying developmental potential. Analysis of egg transcriptome after natural ovulation (4 samples), photoperiod-controlled ovulation (14 samples), and hormonally-induced ovulation (11 samples).

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