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Oryzias

ABSTRACT: ddRAD sequencing of four Oryzias species endemic to Sulawesi

PROVIDER: PRJDB12774 | ENA |

REPOSITORIES: ENA

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Dataset's files

Source:

			Action	DRS
	DRR336048.fastq.gz	Fastqsanger.gz
	DRR336049.fastq.gz	Fastqsanger.gz
	DRR336050.fastq.gz	Fastqsanger.gz
	DRR336051.fastq.gz	Fastqsanger.gz
	DRR336052.fastq.gz	Fastqsanger.gz

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Project description:The aim of this sequencing experiment was to make available tissue expression panels for selected fish species for comparative expression studies between the species. Tissue samples were collected for zebrafish (Danio rerio), medaka (Oryzias latipes), and rainbow trout (Oncorhynchus mykiss). Tissue types included liver, skin, muscle, heart, gut, gill, eye, brain for all three species, with additionally pyloric caeca, kidney, head kidney, and spleen for rainbow trout. Only liver samples were taken in replicate of four or three for rainbow trout. All fish were raised under standard rearing conditions for the species. Total RNA was extracted from the tissue samples and paired‐end sequencing of sample libraries was completed on an Illumina HiSeq 2500 with 125‐bp reads. Processed count tables per species as raw counts, FPKM, or TPM, were generated from read alignment to the Ensembl genomes of the respective species using STAR and gene level counting using RSEM and Ensembl gene annotation.

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