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Oryza sativa Japonica Group

ABSTRACT: RNA-Seq reads of rice shoot and root under 1.6mM (normal) and 0.4mM (low) nitrogen (NH4+) conditions

PROVIDER: PRJDB13104 | ENA |

REPOSITORIES: ENA

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Dataset's files

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			Action	DRS
	DRR351110.fastq.gz	Fastqsanger.gz
	DRR351111.fastq.gz	Fastqsanger.gz
	DRR351112.fastq.gz	Fastqsanger.gz
	DRR351113.fastq.gz	Fastqsanger.gz
	DRR351114.fastq.gz	Fastqsanger.gz

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Oryza sativa Japonica Group

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Project description:Oryza sativa cv. Nipponbare was engineered to over-express a barley alanine aminotransferase (alaAT) gene using the promoter (OsANT1) from a rice aldehyde dehydrogenase gene that expresses in roots. We use biotechnology to improve the nitrogen use efficiency of rice by over-expressing alaAT in a tissue specific (root) manner. The AlaAT enzyme is a reversible aminotransferase that is linked to both C and N metabolism since it uses pyruvate plus glutamate to produce alanine and 2-oxoglutarate, and visa versa. Transcriptome data from the roots and shoots of rice plants at maximum tillering, grown hydroponically on either 0.5, 2 or 5 mM NH4+ as the nitrogen source. Wildtype rice (Nipponbare) and two independent OsANT1:HvAlaAT rice transgenic lines (AGR1/7, and AGR3/8) were grown hydroponically with either 0.5, 2 or 5mM NH4+ as the nitrogen source, to the reproductive stage. Tissue samples were taken at active and maximum tillering from root and shoot, at mid-day of the plants' day/night cycle. The RNA from root and shoot at maxiumum tillering was used for mcroarray analysis. Please read Beatty et al., 2009, PLant Biotechnology Journal 7, pp562-576 for detailed about these transgenic lines. The results from this variable N study were reported in a manuscript submitted to Botany, July 2013

2013-10-25 | E-GEOD-48549 | biostudies-arrayexpress

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Project description:The RNA-seq technique aims to identify genes that are differently expressed in low nitrogen (2mM (NH4)2SO4) and high nitrogen (10mM (NH4)2SO4) conditions. Two groups of Serratia sp. ZM were analysed by us, low nitrogen group and high nitrogen group.

2015-11-01 | E-GEOD-72965 | biostudies-arrayexpress

Oryza sativa Japonica Group

Project description:Gene expression profile of rice shoot and root in response to nitrogen deficiency treatment under phosphorus deficiency condition.

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Nitrogen-efficient rice over-expressing alanine aminotransferase

Project description:Oryza sativa cv. Nipponbare was engineered to over-express a barley alanine aminotransferase (alaAT) gene using the promoter (OsANT1) from a rice aldehyde dehydrogenase gene that expresses in roots. We are using biotechnology to improve the nitrogen use efficiency of rice by over-expressing alaAT in a tissue specific (root) manner. The AlaAT enzyme is a reversible aminotransferase that is linked to both C and N metabolism since it uses pyruvate plus glutamate to produce alanine and 2-oxoglutarate, and visa versa. Wildtype rice (Nipponbare) and three independent OsANT1:HvAlaAT rice transgenic lines (AGR1/7, AGR1/8 and AGR3/8) were grown hydroponically with 5mM NH4+ as the nitrogen source, to the reproductive stage. RNA samples were taken at active tillering, maximum tillering and end-of-tillering stages from root and shoot, at mid-day of the plants' day/night cycle. The RNA from root and shoot at maxiumum tillering was used for microarray analysis. Please read Beatty et al., 2009, Plant Biotechnology Journal 7, pp562-576 for further details..

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