Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Schizosaccharomyces pombe 927

ABSTRACT: The mechanism of universal replication initiation dependent on G4/RNA-DNA hybrids.

PROVIDER: PRJDB13623 | ENA |

REPOSITORIES: ENA

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
	DRR464395_1.fastq.gz	Fastqsanger.gz
	DRR464395_2.fastq.gz	Fastqsanger.gz
	DRR464396_1.fastq.gz	Fastqsanger.gz
	DRR464396_2.fastq.gz	Fastqsanger.gz
	DRR464397_1.fastq.gz	Fastqsanger.gz

Items per page:

1 - 5 of 36

Similar Datasets

Schizosaccharomyces pombe 927

Project description:Schizosaccharomyces pombe 927 Epigenomics

| PRJNA182496 | ENA

Schizosaccharomyces pombe 927

Project description:Identification of synthetic lethal mutation with pot1 deletion

| PRJDB6467 | ENA

Schizosaccharomyces pombe 927

Project description:Schizosaccharomyces pombe 927 Epigenomics

| PRJNA182498 | ENA

Schizosaccharomyces pombe

Project description:Multiple bypass pathways of Hsk1 kinase essential for initiation of DNA replication in fission yeast

| PRJNA145303 | ENA

The essential Schizosaccharomyces pombe Pfh1 DNA helicase promotes fork movement past G-quadruplex motifs to prevent DNA damage

Project description:Background G-quadruplexes (G4s) are stable non-canonical DNA secondary structures consisting of stacked arrays of four guanines, each held together by Hoogsteen hydrogen bonds. Sequences with the ability to form these structures in vitro, G4 motifs, are found throughout bacterial and eukaryotic genomes. The budding yeast Pif1 DNA helicase, as well as several bacterial Pif1 family helicases, unwind G4 structures robustly in vitro and suppress G4-induced DNA damage in S. cerevisiae in vivo. Results We determined the genomic distribution and evolutionary conservation of G4 motifs in four fission yeast species and investigated the relationship between G4 motifs and Pfh1, the sole S. pombe Pif1 family helicase. Using chromatin immunoprecipitation combined with deep sequencing, we found that many G4 motifs in the S. pombe genome were associated with Pfh1. Cells depleted of Pfh1 had increased fork pausing and DNA damage near G4 motifs, as indicated by high DNA polymerase occupancy and phosphorylated histone H2A, respectively. In general, G4 motifs were underrepresented in genes. However, Pfh1-associated G4 motifs were located on the transcribed strand of highly transcribed genes significantly more often than expected, suggesting that Pfh1 has a function in replication or transcription at these sites. Conclusions In the absence of functional Pfh1, unresolved G4 structures cause fork pausing and DNA damage of the sort associated with human tumors.

2014-12-03 | GSE59178 | GEO

Schizosaccharomyces pombe

Project description:Replication stress affects epigenetic stability

| PRJNA353373 | ENA

Schizosaccharomyces pombe

Project description:Arrested replication forks guide retrotransposon integration

| PRJNA280702 | ENA

Schizosaccharomyces pombe

Project description:A global profile of replication polymerase usage

| PRJNA263280 | ENA

Schizosaccharomyces pombe

Project description:The histone variant H2A.Z promotes initiation of meiotic recombination (expression)