Project description:PacBio HiFi sequencing was used to characterize genome-wide DNA base modifications and methylation motifs in Alicyclobacillus acidocaldarius subsp. acidocaldarius strain KCTC 1825 (DSM 446). Base-modification kinetics were mapped to the complete genome assembly and analyzed for m6A and m4C signals. The processed dataset provides per-base modification calls, motif-associated calls, and motif summaries linked to the public genome assembly and SRA run.
Project description:Alicyclobacillus acidoterrestris and Alicyclobacillus suci are both guaiacol producers that can cause spoilage in products like juices. To study the genetic underpinnings of guaiacol production, transcriptomics was performed on multiple strains of A. suci and A. acidoterrestris under guaiacol production conditions in apple juice. While guaiacol production was consistently higher by A. acidoterrestris than by A. suci, the gene expression levels associated with guaiacol production (e.g., vdcC, vdcB, vdcD) showed significant variability across strains of both species, suggesting that other factors beyond vdcC transcription influence the amount of guaiacol produced. This research provides insights for beverage producers in assessing spoilage risks and developing more informed mitigation strategies.