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Daphnia magna

ABSTRACT: Analysis of sex-specific isoform expression in the crustacean Daphnia magna

PROVIDER: PRJDB13788 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	DRR383806_subreads.fastq.gz	Fastqsanger.gz
	DRR383807_subreads.fastq.gz	Fastqsanger.gz
	DRR383808.fastq.gz	Fastqsanger.gz
	DRR383809_subreads.fastq.gz	Fastqsanger.gz
	DRR383810_subreads.fastq.gz	Fastqsanger.gz

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Transcriptional atlas of Daphnia magna

Project description:Transcriptomics studies are more likely to achieve predictive results when they rely on tissue- and cell-specific transcriptional data. Identification of cell types in novel model organisms by their transcriptional profiles is difficult without data on transcriptional differences among major tissues and anatomical features. Here we report the first dataset on tissue- and organ-specific transcriptomics in freshwater plankton crustacean Daphnia magna, reporting markers of eggs, hemocytes, gut, carapace, antennae and head (as well as the remaining carcass).

2024-04-19 | GSE264123 | GEO

D. magna CT448 protease specificity

Project description:Mass developments of toxin-producing cyanobacteria are frequently observed in freshwater ecosystems due to eutrophication and global warming. These mass developments can partly be attributed to cyanobacterial toxins, such as protease inhibitors (PIs), which inhibit digestive serine proteases of Daphnia, the major herbivore of phytoplankton and cyanobacteria. To date, mechanisms of this inhibition in the gut of the crustacean Daphnia magna are not known. Here, we characterize a single serine protease, chymotrypsin 448 (CT448), which is present in the gut of the crustacean D. magna.

2018-10-04 | PXD010978 | Pride

Daphnia magna exposed to narcotics and polar narcotics

Project description:This SuperSeries is composed of the following subset Series: GSE29854: Daphnia magna exposed to narcotics and polar narcotics - aniline GSE29856: Daphnia magna exposed to narcotics and polar narcotics - 4-chloroaniline GSE29857: Daphnia magna exposed to narcotics and polar narcotics - 3,5-dichloroaniline GSE29858: Daphnia magna exposed to narcotics and polar narcotics - 2,3,4-trichloroaniline GSE29862: Daphnia magna exposed to narcotics and polar narcotics - ethanol GSE29864: Daphnia magna exposed to narcotics and polar narcotics - isopropanol GSE29867: Daphnia magna exposed to narcotics and polar narcotics - methanol Refer to individual Series

2012-02-01 | E-GEOD-29993 | biostudies-arrayexpress

Daphnia magna Single Cell Atlas

Project description:This is the first view of Daphnia single-cell transcriptomics-based cell atlas. Daphnia is a freshwater plankton crustacean which is both a classic and emerging new model for eco-physiology, toxicology, and evolutionary genomics. We were able to identify over 30 distinct cell types about half of which could be functionally annotated.

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Two Doublesex1 mutants revealed a tunable gene network underlying intersexuality in Daphnia magna

Project description:Purpose: To justify whether sex is strictly binary or variable along a spectrum, exhaustive investigation into intersexuality is necessary. In this study, by mutating the sex determining locus Doublesex1, we generated two Daphnia magna strains from which two different forms of feminized males could be obtained. mRNA sequencing of these intersex males was carried out to explore the transcritomic shift responsible for the changes in sexual phenotype. Methods: Two Doublesex1 mutants, namely Line A and Line B, were generated by microinection of designed TALENs into Daphnia magna eggs. Line A males showed severe, while Line B males showed minor bodywide feminization. Whole body mRNA profiles of 40 hour post oviposition embryos were generated for wildtype female, Line A male, Line B male and wildtype male using Illumina sequencing (mRNA sequencing service provided by Novogene). Data analysis was done using CLC Genomics Workbench software. Results: Among 731 sex-biased genes identified in this dataset, more than half (449 genes) showed non-binary expression profile in which their expression in the mutant males was at an intermediate level between wildtype female and wildtype male. Conclusions: Downstream genes of Doublesex1 cascade may fluctuate in activity and give rise to a spectrum of sexual phenotypes in Daphnia magna.

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RNA-sequencing analysis of genetically identical female and male Daphnia magna with age

Project description:Comparison of female and male Daphnia magna gene expression with age. The sexes in Daphnia magna are genetically identical. The aim of this study was to identify possible differences in gene expression between genders with age.

2020-09-23 | GSE125466 | GEO

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Project description:Daphnia magna Transcriptome or Gene expression

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Daphnia magna

Project description:Daphnia magna Transcriptome or Gene expression

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Energy starvation in Daphnia magna from exposure to a lithium cobalt oxide nanomaterial

Project description:We determined lithium cobalt oxide LCO’s effects on pathways in the model organism Daphnia magna through RNA-Seq global gene expression analysis.

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