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Homo sapiens

ABSTRACT: RNA-seq analysis of endometrial epithelial cells exposed to pro-inflammatory cytokines

PROVIDER: PRJDB13951 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	DRR395305_1.fastq.gz	Fastqsanger.gz
	DRR395305_2.fastq.gz	Fastqsanger.gz
	DRR395306_1.fastq.gz	Fastqsanger.gz
	DRR395306_2.fastq.gz	Fastqsanger.gz
	DRR395307_1.fastq.gz	Fastqsanger.gz

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Project description:A Mechanistic Analysis of the Role of Pro-inflammatory Cytokines in Induction of Epithelial to Mesenchymal Transition in Hepatocellular Carcinoma

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Red Algae-Derived Mineral Intervention to Counter Pro-inflammatory Activity in Human Colon Organoids

Project description:Human colon organoids were maintained in culture medium alone or exposed to lipopolysaccharide with a combination of three pro-inflammatory cytokines (tumor necrosis factor-a, interleukin-1b and interferon-g [LPS-cytokines]) to mimic the environment in the inflamed colon. Untreated organoids and those exposed to LPS-cytokines were concomitantly treated with a multi-mineral product that has previously been shown to improve barrier structure/function. The organoids were subjected to proteomic analysis to obtain a broad view of the protein changes induced by these interventions. In parallel, confocal fluorescence microscopy and trans-epithelial electrical resistance measurements were used to assess barrier structure/function. The LPS-cytokines altered expression of multiple proteins that influence innate immunity and promote inflammation. Most of these were unaffected by the multi-mineral intervention, though a subset of inflammation-related proteins including fibrinogen-b and -g chains, phospholipase A2 and SPARC was down-regulated in the presence of the multi-mineral intervention; another subset of proteins with anti-inflammatory, antioxidant or anti-microbial activity was up-regulated by multi-mineral treatment. When used alone, the multi-mineral intervention strongly up-regulated proteins that contribute to barrier formation and tissue strength. Concomitant treatment with LPS-cytokines did not inhibit barrier formation in response to the multi-mineral intervention.

2023-06-30 | PXD038941 | Pride

Endometrial epithelial cell transcriptome response to co-culture with adipose stromal cells

Project description:The role of obesity in endometrial cancer development is tested by co-culturing adipose stromal cells (ASCs) with endometrial epithelial cells and endometrial cancer cell Ishikawa for 21 days. Control cells (not exposed to ASCs) were incubated for the same duration. RNA-seq identified differential expression due to ASC exposure

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Pro-Inflammatory Cytokines Mediate the Epithelial-to-Mesenchymal-Like Transition of Pediatric Posterior Fossa Ependymoma (single-nucleus RNA-seq)

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Project description:RNA-seq analysis of immortalized human endometrial stromal cells exposed to cigarette smoke extracts

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Homo sapiens

Project description:Protein phosphorylations, mediated by pro-inflammatory cytokines IL-32g and IL-17A/F

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Corticosteroid Sensitivity and the Effect of Th1 and Th17 Cytokines on Gene Expression in Human Airway Smooth Muscle

Project description:Purpose: Using RNA-sequencing, determine the effect of corticosteroids on human airway smooth muscle cells (ASM) exposed to Th1 and/or Th17 cytokines. Effects of cytokines and/or corticosteroids on gene expression was assessed. Methods: Pediatric human ASM (ages 0-21; n=4/treatment group) were serum starved for 48 h and then treated with 10 ng/mL TNFα, IFNγ, IL-17A, TNFα/IFNγ, or IFNγ/IL-17A in the presence of vehicle (0.01% DMSO) or 10 nM fluticasone propionate (FP). Cells were harvested after 18 h for RNA isolation, QC, library prep, and RNA sequencing on the Illumina HiSeq4000 platform. RNA quality and integrity was determined by gel electrophoresis and Agilent 2100 Bioanalyzer. Following sequencing. FASTQ files were processed and analyzed to generate counts files and perform differential expression analysis. Results: Our analyses show that cytokines, particularly TNFα, IFNγ, and TNFα/IFNγ had significant effects on gene expression. Differentially expressed genes were largely related to pro-inflammatory responses. Interestingly, we found that IL-17A had only modest effects on gene expression. Corticosteroids (FP) reduced or inhibited the effects of pro-inflammatory cytokines. However, these anti-inflammatory effects were reduced in cells treated with TNFα/IFNγ or IL-17A/IFNγ. Conclusions: These studies highlight the differential effects of pro-inflammatory cytokines and corticosteroids on gene expression in human ASM.

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