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Brassica oleracea var. capitata strain:Rinen

ABSTRACT: RNA-seq of cabbage

PROVIDER: PRJDB14757 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	DRR426048_1.fastq.gz	Fastqsanger.gz
	DRR426048_2.fastq.gz	Fastqsanger.gz
	DRR426049_1.fastq.gz	Fastqsanger.gz
	DRR426049_2.fastq.gz	Fastqsanger.gz
	DRR426050_1.fastq.gz	Fastqsanger.gz

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Project description:Cabbage belonging to Brassicaceae family is one of the most important vegetables cultivated worldwide. The economically important part of cabbage crop is head, formed by leaves which may be of splitting and non-splitting types. Cabbage varieties showing head splitting causes huge loss to the farmers and therefore finding the molecular and structural basis of splitting types would be helpful to breeders. To determine which anatomical characteristics were related to head-splitting in cabbage, we analyzed two contrasting cabbage lines and their offspring using a field emission scanning electron microscope. The inbred line "747" is an early head-splitting type, while the inbred line "748" is a head-splitting-resistant type. The petiole cells of "747" seems to be larger than those of "748" at maturity; however, there was no significant difference in petiole cell size at both pre-heading and maturity stages. The lower epidermis cells of "747" were larger than those of "748" at the pre-heading and maturity stages. "747" had thinner epidermis cell wall than "748" at maturity stage, however, there was no difference of the epidermis cell wall thickness in the two lines at the pre-heading stage. The head-splitting plants in the F1 and F2 population inherited the larger cell size and thinner cell walls of epidermis cells in the petiole. In the petiole cell walls of "747" and the F1 and F2 plants that formed splitting heads, the cellulose microfibrils were loose and had separated from each other. These findings verified that anomalous cellulose microfibrils, larger cell size and thinner-walled epidermis cells are important genetic factors that make cabbage heads prone to splitting.

| S-EPMC4633201 | biostudies-literature

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