Project description:Two new species of forget-me-nots, Myosotis chaffeyorum C.A.Lehnebach sp. nov. and Myosotis mooreana C.A.Lehnebach sp. nov. are described and illustrated. These species are endemic to New Zealand and restricted to the mountains of north-west Nelson (South Island). Both species are uncommon and Myosotis mooreana is known from the type locality only. Their conservation status is rated as "Nationally Critical". A table listing differences between these two new species and other similar species and an identification key are provided.
| S-EPMC3492931 | biostudies-literature
Project description:chloroplast genome of Lappula myosotis
Project description:Lappula myosotis V. Wolf 1776 is an annual or biennial plant with important medicinal value. In the present study, we report the complete chloroplast genome data of L. myosotis, which has a length of 146,668 bp, including a small single-copy (SSC) region of 17,059 bp, a large single-copy (LSC) region of 79,691 bp, and a pair of inverted repeats (IRs) of 24,959 bp. A total of 127 genes encoding tRNA and rRNA were annotated. The total CG content of the chloroplast genome was 37.7%. The maximum-likelihood (ML) phylogenetic tree strongly supported that L. myosotis is closely related to Trigonotis peduncularis. The complete chloroplast genome of L. myosotis provides useful information on the evolution and phylogenetic relationship among Boraginaceae plants.
Project description:Microsatellite loci were developed as polymorphic markers for the New Zealand endemic Myosotis pygmaea species group (Boraginaceae) for use in species delimitation and population and conservation genetic studies. Illumina MiSeq sequencing was performed on genomic DNA from seedlings of M. drucei. From trimmed paired-end sequences >400 bp, 484 microsatellite loci were identified. Twelve of 48 microsatellite loci tested were found to be polymorphic and consistently scorable when screened on 53 individuals from four populations representing the geographic range of M. drucei. They also amplify in all other species in the M. pygmaea species group, i.e., M. antarctica, M. brevis, M. glauca, and M. pygmaea, as well as 18 other Myosotis species. These 12 polymorphic microsatellite markers establish an important resource for research and conservation of the M. pygmaea species group and potentially other Southern Hemisphere Myosotis.
