Project description:We sought to determine how a cystic fibrosis isolate of Stenotrophomonas maltophilia responds to relevant pH gradients (pH 5, 7, and 9) by growing the bacterium in phosphate buffered media and conducting RNAseq experiments. Our data suggests acidic conditions are stressful for strain FLR19, as it responded by increasing expression of stress-response and antibiotic-resistance genes.
Project description:S. maltophilia was exposed to a simulated microgravity (SMG) environment in high-aspect ratio rotating-wall vessels bioreactors for 14 days, while the control group was performed in the same bioreactors under normal gravity (NG) environment. After that, combined phenotypic, genomic, transcriptomic and proteomic analyses were conducted to compare the influence of the SMG and NG on S. maltophilia.