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Organismal metagenomes

ABSTRACT: Shotgun metagenome sequencing of oral and stool samples from Japanese long-term care facility residents

PROVIDER: PRJDB16443 | ENA |

REPOSITORIES: ENA

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Dataset's files

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			Action	DRS
	DRR502069_1.fastq.gz	Fastqsanger.gz
	DRR502069_2.fastq.gz	Fastqsanger.gz
	DRR502070_1.fastq.gz	Fastqsanger.gz
	DRR502070_2.fastq.gz	Fastqsanger.gz
	DRR502071_1.fastq.gz	Fastqsanger.gz

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Project description:Metagenome assembly of PRJDB16443 data set (Shotgun metagenome sequencing of oral and stool samples from Japanese long-term care facility residents)

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Staphylococcus aureus

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Exploring the effect and mechanism of roselle drink on the urinary tract infection in clinics and the lipopolysaccharide-induced renal inflammation in mice

Project description:Urinary tract infection (UTI) is a common problem in long-term care facilities. Roselle (Hibiscus sabdariffa Linn.) has been used in fold medicine as an anti-inflammatory agent. In this study, we surveyed the effect of roselle drink on the prevention of UTI in long-term care facilities and analyzed the anti-inflammatory potential of roselle on lipopolysaccharide (LPS)-induced renal inflammation. By survey questionnaires, we found that roselle drink was the most commonly used treatment for the routine care of residents. In addition, taking roselle drink in residents with urinary catheters reduced the incidence of UTI by 36%. Roselle suppressed LPS-induced nuclear factor-κB (NF-κB) activation in cells in a dose-dependent manner, and the maximal inhibition (73.75±4.11%) was observed at 100 μg/ml roselle drink. Roselle also suppressed LPS-induced interleukin-1β (IL-1β) production in mice. Gene expression profile of roselle in kidney showed that roselle downregulated the expression of inflammatory genes, and NF-κB was the main transcription factor involved in the regulation of roselle-regulated gene expression. Immunohistochemical staining further showed that roselle inhibited LPS-induced NF-κB activation and inflammatory cell infiltration in kidney. In conclusion, our findings suggested that roselle drink might be a potent benefit herbal supplement for UTI. Moreover, roselle ameliorated LPS-induced renal inflammation via regulating inflammatory gene expression and NF-κB pathway.

2017-08-21 | GSE73939 | GEO

Pseudomonadota

Project description:AMR bacterial strains in Japanese long-term-care facilities

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Gammaproteobacteria

Project description:AMR bacterial strains in Japanese long-term-care facilities

| PRJDB12072 | ENA