Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Ralstonia pseudosolanacearum strain:OE1-1

ABSTRACT: Transcriptome analysis of signal transduction pathway in quorum sensing in Ralstonia pseudosolanacerum strain OE1-1

PROVIDER: PRJDB16932 | ENA |

REPOSITORIES: ENA

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
	DRR512175_1.fastq.gz	Fastqsanger.gz
	DRR512175_2.fastq.gz	Fastqsanger.gz
	DRR512176_1.fastq.gz	Fastqsanger.gz
	DRR512176_2.fastq.gz	Fastqsanger.gz
	DRR512177_1.fastq.gz	Fastqsanger.gz

Items per page:

1 - 5 of 37

Similar Datasets

Ralstonia pseudosolanacearum strain:OE1-1

Project description:Transcriptome analysis of quorum sensing genes in Ralstonia pseudosolanacerum strain OE1-1

| PRJDB15428 | ENA

Ralstonia pseudosolanacearum strain:OE1-1

Project description:Transcriptome analysis of downstream genes of quorum sensing in Ralstonia pseudosolanacerum strain OE1-1

| PRJDB18011 | ENA

Ralstonia pseudosolanacearum strain:OE1-1

Project description:Transcriptome analysis of siderophore production-related genes in Ralstonia pseudosolanacerum strain OE1-1

| PRJDB16234 | ENA

Ralstonia solanacearum OE1-1

Project description:Complete genome sequence of Ralstonia solanacearum OE1-1

| PRJDB4012 | ENA

Ralstonia pseudosolanacearum

Project description:Ralstonia pseudosolanacearum overview

| PRJNA398430 | ENA

Ralstonia pseudosolanacearum

Project description:Ralstonia pseudosolanacearum Raw sequence reads

| PRJNA1271622 | ENA

Ralstonia pseudosolanacearum strain:OE1-1

Project description:Transcriptome analysis of plant cell wall degradation enzyme coding gene

| PRJDB15155 | ENA

The transcription regulator ChpA affects the global transcriptome including quorum sensing-dependent genes in Ralstonia pseudosolanacearum strain OE1-1.

Project description:The gram-negative plant-pathogenic β-proteobacterium Ralstonia pseudosolanacearum strain OE1-1 produces methyl 3-hydroxymyristate as a quorum sensing (QS) signal through methyltransferase PhcB and senses the chemical via the sensor histidine kinase PhcS. This leads to activation of the LysR family transcription regulator PhcA, which regulates the genes (QS-dependent genes) responsible for QS-dependent phenotypes, including virulence. The transcription regulator ChpA, which possesses a response regulator receiver domain and also a hybrid sensor histidine kinase/response regulator phosphore-acceptor domain but lacks a DNA-binding domain, is reportedly involved in QS-dependent biofilm formation and virulence of R. pseudosolanacearum strain GMI1000. To explore the function of ChpA in QS of OE1-1, we generated a chpA-deletion mutant (ΔchpA) and revealed that the chpA deletion leads to significantly altered QS-dependent phenotypes. Furthermore, ΔchpA exhibited a loss in its infectivity in xylem vessels of tomato plant roots, losing virulence on tomato plants, similar to the phcA-deletion mutant (ΔphcA). Transcriptome analysis showed that the transcript levels of phcB, phcQ, phcR, and phcA in ΔchpA were comparable to those in OE1-1. However, the transcript levels of 89.9% and 88.9% of positively and negatively QS-dependent genes, respectively, were significantly altered in ΔchpA compared with OE1-1. Furthermore, the transcript levels of these genes in ΔchpA were positively correlated with those in ΔphcA. Together, our results suggest that ChpA is involved in the regulation of these QS-dependent genes, thereby contributing to the behaviour in host plant roots and virulence of OE1-1.

| S-EPMC10576176 | biostudies-literature

Ralstonia pseudosolanacearum GMI1000

Project description:Ralstonia solanacearum Tn-seq raw reads

| PRJNA603426 | ENA

Ralstonia pseudosolanacearum GMI1000

Project description:Ralstonia solanacearum, genomic and transcriptomic modifications

| PRJNA644288 | ENA