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Bos taurus

ABSTRACT: Effects of intrauterine extracellular vesicle microRNAs on embryonic gene expression in low-fertility cows

PROVIDER: PRJDB18321 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	DRR574542_1.fastq.gz	Fastqsanger.gz
	DRR574542_2.fastq.gz	Fastqsanger.gz
	DRR574543_1.fastq.gz	Fastqsanger.gz
	DRR574543_2.fastq.gz	Fastqsanger.gz
	DRR574544_1.fastq.gz	Fastqsanger.gz

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Project description:Effects of intrauterine extracellular vesicle microRNAs on embryonic gene expression in low-fertility cows

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Differential expression profiles of extracellular vesicle-coupled miRNA in follicular fluid of cows at different metabolic status

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SWATH-MS analysis of blood plasma and circulating small extracellular vesicles enables detection of putative protein biomarkers of fertility in Young and Aged dairy cows.

Project description:The development of biomarkers of fertility could provide benefits for the genetic improvement of dairy cows. Circulating small extracellular vesicles (sEVs) show promise as diagnostic or prognostic markers since their cargo reflects the metabolic state of the cell of origin; thus, they mirror the physiological status of the host. Here, we employed data-independent acquisition mass spectrometry to survey the plasma and plasma sEV proteomes of two different cohorts of Young (Peripubertal; n = 30) and Aged (Primiparous; n = 20) dairy cows (Bos taurus) of high- and low-genetic merit of fertility and known pregnancy outcomes (ProteomeXchange dataset identifier PXD042891). We established predictive models of fertility status, with an area under the curve of 0.97 (sEV; p value = 3.302e-07) and 0.95 (plasma; p value = 6.405e-08). Biomarker candidates unique to high-fertility Young cattle had a sensitivity of 0.77 and specificity of 0.67 (*p = 0.0287). Low-fertility biomarker candidates uniquely identified in sEVs from Young and Aged cattle had a sensitivity and specificity of 0.69 and 1.0, respectively (***p = 0.0005). Our bioinformatics pipeline enabled quantification of plasma and circulating sEV proteins associated with fertility phenotype. Further investigations are warranted to validate this research in a larger population, which may lead to improved classification of fertility status in cattle.

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