Project description:The present study examines changes in global gene expression patterns and in virulence factor-associated genes in an extended spectrum beta-lactamase (ESBL)-producing UPEC (ESBL019) during the morphologic transitions induced by an ineffective antibiotic and in the presence of human primary bladder epithelial cells. The morphological shifts induced by ineffective antibiotics are associated with significant transcriptional virulence alterations in ESBL-producing UPEC, which may affect survival and persistence in the urinary tract.
Project description:This project presents LC-MS/MS-based proteomic datasets obtained from bacterial colonies isolated from retail chicken meat samples, as part of a study focused on the detection of Campylobacter jejuni. The isolates were initially screened using a novel chromogenic enzymatic assay targeting hippuricase activity, which is specific for C. jejuni. Selected samples, including both enzymatically positive and negative results, were subjected to in-gel digestion followed by high-resolution LC-MS/MS analysis on an Orbitrap Exploris 240 instrument.
