Project description:We used the bioluminescent squid symbiont, Vibrio fischeri, to identify essential regulatory factors that control expression and function of a strain-specific T6SS encoded within a genomic island. Random transposon mutagenesis revealed that three genes located on the T6SS2-encoding genomic island are necessary to activate expression of a T6SS reporter. We used a proteomics approach to identify proteins that were differentially abundant in mutant strains compared to the wild type when cells were grown in a high-viscosity media.
Project description:Folsomia candida (Collembola) is able to survive dryer conditions by absorbing water vapour from its surroundings. To unravel the genomic responses underlying this intriguing water-absorption mechanism, we exposed the species to 98.2% Relative Humidity (eight, 27, 53 and 174 hours respectively) and subjected it to microarray based transcription profiling.
Project description:Here, we report genome sequences of the two bioluminescent S. aureus strains Xen31 and Xen36, obtained from PerkinElmer (#119242 and #119243, respectively). Xen31 was derived from the parental MRSA strain ATCC33591, a clinical strain isolated at Elmhurst Hospital in New York City. Xen36 was derived from parental strain ATCC 49525, a clinical isolate from a bacteremic patient. A copy of the modified luxABCDE operon from Photorhadbus luminescenst is integrated in the chromosome of Xen31 and in a native plasmid of Xen36.