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Cupriavidus necator H16

ABSTRACT: A promoter library for tuning gene expression in Cupriavidus necator under autotrophic conditions

PROVIDER: PRJDB19890 | ENA |

REPOSITORIES: ENA

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Dataset's files

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			Action	DRS
	DRR628345_1.fastq.gz	Fastqsanger.gz
	DRR628345_2.fastq.gz	Fastqsanger.gz
	DRR628346_1.fastq.gz	Fastqsanger.gz
	DRR628346_2.fastq.gz	Fastqsanger.gz
	DRR628347_1.fastq.gz	Fastqsanger.gz

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Project description:These data belong to a metabolic engineering project that introduces the reductive glycine pathway for formate assimilation in Cupriavidus necator. As part of this project we performed short-term evolution of the bacterium Cupriavidus necator H16 to grow on glycine as sole carbon and energy source. Some mutations in a putiative glycine transporting systems facilitated growth, and we performed transcriptomics on the evolved strain growing on glycine. Analysis of these transcriptomic data lead us to the discovery of a glycine oxidase (DadA6), which we experimentally demonstrated to play a key role in the glycine assimilation pathay in C. necator.

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Investigation of protein allocation and utilization in the versatile chemolithoautotroph Cupriavidus necator

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Comparative RNA-Seq of Cupriavidus necator H16, formerly Ralstonia eutropha H16, grown in minimal media with 3-hydroxypropionic acid (3-HP) or fructose as sole carbon source.

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