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Cuscuta campestris

ABSTRACT: Cuscuta_transcriptome

PROVIDER: PRJDB19976 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	DRR689958_1.fastq.gz	Fastqsanger.gz
	DRR689958_2.fastq.gz	Fastqsanger.gz
	DRR689959_1.fastq.gz	Fastqsanger.gz
	DRR689959_2.fastq.gz	Fastqsanger.gz
	DRR689960_1.fastq.gz	Fastqsanger.gz

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microRNA accumulation from Cuscuta campestris in vitro haustoria

Project description:The parasitic plant Cuscuta campestris produces specialized microRNAs that are specifically expressed at the haustorial interface. Some of these "Interface-Induced MicroRNAs" function to target host mRNAs. C. campestris haustoria can be induced in the absence of any host tissues using a combination of light and physical pressure. This experiment tested with such in vitro, host-free haustoria produced interface-induced microRNAs. Small RNA-seq was performed from three different treatments: Shoot tips of C. campestris without any haustoria formation, in vitro haustoria formed in the presence of host tissue (detached leaves of Arabidopsis thaliana) and in vitro haustoria formed in the absence of any host tissue.

2022-07-06 | GSE205256 | GEO

Small RNAs from Cuscuta campestris parasitizing Arabidopsis thaliana

Project description:Dodders (Cuscuta spp.) are obligate parasitic plants that obtain water and nutrients from the stems of host plants via specialized feeding structures called haustoria. Dodder haustoria facilitate bi-directional movement of viruses, proteins, and mRNAs between host and parasite, but the functional effects of these movements are not clear. Here we show that C. campestris haustoria accumulate high levels of many novel microRNAs (miRNAs) while parasitizing Arabidopsis thaliana hosts. Many of these miRNAs are 22 nts long, a usually rare size of plant miRNA associated with amplification of target silencing through secondary small interfering RNA (siRNA) production. Several A. thaliana mRNAs are targeted by C. campestris 22 nt miRNAs during parasitism, resulting in high levels of secondary siRNA production. The targeted mRNAs function in hormone perception, pathogen-defense signaling, phloem function, and stem-cell identity. Homologs of these target mRNAs from diverse plants also have high-confidence complementary sites to C. campestris miRNAs, suggesting that homologous mRNAs are targeted by C. campestris across its very broad host range. These data show that C. campestris miRNAs act as trans-species regulators of host gene expression, and suggest that they may act as virulence factors during parasitism.

2017-12-18 | GSE84955 | GEO

Cuscuta campestris

Project description:Parasitism-evoked horizontal gene transfer between plants as a novel trigger for specialized metabolism evolution

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Project description:Cuscuta campestris parasitized on various hosts including bamboo stick, wt Nicotiana tabacum, amiR targeting CcJKD-expressing N. tabacum

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Project description:A host-free transcriptome for early haustorium development in Cuscuta campestris

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Project description:Cuscuta campestris overview

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Cuscuta campestris

Project description:Cuscuta campestris and Cucumis sativus Raw sequence reads

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Project description:transcriptome analysis for haustroium development in Cuscuta campestris parasitizing on host plant A. thaliana

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An efficient Rhizobium rhizogenes-mediated transformation system for Cuscuta campestris.

Project description:Parasitism has evolved independently in various plant families, with Cuscuta campestris (field dodder) being an economically significant example. Despite advances in genomics and transcriptomics, functional studies in C. campestris are limited by the lack of an efficient genetic transformation system. This study introduces a highly effective Rhizobium rhizogenes-mediated transformation system for C. campestris using a pBIN plasmid harboring a Yellow Fluorescence Protein reporter gene. We optimized transformation and regeneration by assessing explant type, media composition, and plant growth regulators. Notably, host plant contact was essential for transgenic shoot regeneration. Over 70% transformation efficiency was achieved using cuttings co-incubated with modified Murashige and Skoog medium and 5 mg/L Benzylaminopurine, followed by transfer to tomato hosts. Additionally, we developed a complete in-vivo protocol over 30% regeneration efficiency. Transgenic shoots were confirmed for rol gene expression and haustoria formation, advancing functional studies in C. campestris.

| S-EPMC11844837 | biostudies-literature

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