Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Drosophila melanogaster

ABSTRACT: Transcriptomics of Gcn5-knocked down larvae and epigenomics of Drosophila melanogaster larvae fed on a diet rich in oleic acid, valine and acetate

PROVIDER: PRJDB20000 | ENA |

REPOSITORIES: ENA

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
	DRR635698.fastq.gz	Fastqsanger.gz
	DRR635699.fastq.gz	Fastqsanger.gz
	DRR635700.fastq.gz	Fastqsanger.gz
	DRR635701.fastq.gz	Fastqsanger.gz
	DRR635702.fastq.gz	Fastqsanger.gz

Items per page:

1 - 5 of 36

Similar Datasets

Drosophila melanogaster

Project description:Transcriptomics of Drosophila melanogaster larvae fed on a diet rich in oleic acid, valine and acetate

| PRJDB18504 | ENA

Dietary enrichments cause alterations in metabolome, transcriptome and improve freeze-tolerance in larvae of the fruit fly (Drosophila melanogaster).

Project description:In this experiment, Drosophila melanogaster larvae were fed with Proline and Arginine compounds and showed a better tolerance to freezing compared to control larvae. Using a microarray approach, we investigated the alteration of transcriptome profiles in larvae fed with Proline or Arginine versus control larvae.

2019-01-01 | E-MTAB-3911 | biostudies-arrayexpress

Comparison of Drosophila melanogaster ovarian transcriptome after germline specific knock down of lid or sin3A genes

Project description:Transcriptomic analysis and identification of differentially expressed genes in wt vs KD Drosophila melanogaster ovaries. We compared gene expression profiles in Drosophila melanogaster ovaries in which the lid or the sin3A gene have been selectively knocked down by tissues specific shRNA expression.

2019-12-11 | GSE133064 | GEO

Comparison of Drosophila melanogaster ovarian transcriptome after germline specific knock down of Snr1 or mod(mdg4) genes

Project description:Transcriptomic analysis and identification of differentially expressed genes in control vs KD Drosophila melanogaster ovaries. We compared gene expression profiles in Drosophila melanogaster ovaries in which the Snr1 or the mod(mdg4) gene have been selectively knocked down by tissues specific shRNA expression.

2021-12-10 | GSE174263 | GEO

Comparison of gene regulation by Gcn5 under KCl stress in S. cerevisiae and S. pombe

Project description:This is parallel comparison of gene regulation by Gcn5 in evolutionarily divergent yeasts S. pombe and S. cerevisiae. Our study showed Gcn5 is required for a similar spectrum of stress responses in both organisms, including the response to KCl. This DNA microarray studies is to find conserved or diverged gene regulation pattern under KCl stress condition in the two yeasts. There are 6 subsets in total, 3 subsets in each evolutionarily divergent yeasts including the following: 1 gcn5 mutant compared to wt under rich medium without treatment, 2 gcn5 mutant compared to wild type both under KCl treatment, 3 wild type strains under KCl treatment compared to wild type without treatment. Keywords: stress response, Gcn5, Comparative genomics, Transcriptional co-activator Gene expression profiling experiment in which gene expression in logarithmically growing cultures of test groups was compared to that of the control groups in rich medium at 25C. There are 6 subsets in total, 3 subsets in each yeast including the following: 1 gcn5 mutant compared to wt under rich medium without treatment, 2 gcn5 mutant compared to wild type both under KCl treatment, 3 wild type strains under KCl treatment compared to wild type without treatment. RNA was extracted and subjected to cDNA expression profiling analysis using the established protocols (Xue et al, 2004). Independent RNA preparations were used to hybridize to microarray slides with dye swap. Data normalized with 'Lowess' per chip per spot normalization.

2010-06-24 | E-GEOD-5218 | biostudies-arrayexpress

Glucocorticoid recptor knockdown in primary amnion epithelial cells treated with MPA and TNFa

Project description:To analyze the effect of glucocorticoids on primary amnion epthelial cells, we harvested tissue samples from 10 healthy ceseaean section patients. We knocked down the glucocorticoid receptor with an siRNA or used a control siRNA. We treated samples with medroxyprogesterone acetate. Half the cells we subjected to a TNFa challenge

2022-04-09 | GSE200312 | GEO

Sequencing of the transcriptome from embryonic and larval hemocytes in gcm knock down background

Project description:Hemocytes were sorted from stage 16 embryos and wandering 3rd instar larvae in which gcm was knocked down using gcmRNAi and the driver srp(hemo)Gal4. The experiments were done in triplicate. The wild type controls at stage 16 embryo and wandering larvae are available under this assession number: E-MTAB-8702.

2022-10-18 | E-MTAB-11966 | biostudies-arrayexpress

Profiling of gut gene expression in Cry1Ab-resistant and susceptible strains of Ostrinia nubilalis reveals possible adaptations of resistant larvae to transgenic Cry1Ab corn

Project description:We revealed a total of 398 gut genes differentially expressed (i.e., either up- or down-regulated genes with expression ratio ≥2.0) in S-strain larvae, while only 264 gut genes differentially expressed in R-strain larvae after fed transgenic corn leaves. Although the percentages of down-regulated genes among the total number of differentially expressed genes (199 genes or 50% in S-strain; 119 genes or 45% in R-strain) were similar between the R and S-strains, the expression ratios of down-regulated genes were much higher in S-strain than in R-strain. These results imply that R-strain larvae had an increased ability to deal with transgenic corn as compared with S-strain larvae. We also revealed that 17 and 9 significantly up- or down-regulated gut genes from S- and R-strain larvae, respectively, including serine proteases (trypsins and chymotrypsins) and aminopeptidases.

2016-09-28 | GSE87393 | GEO

POS-1 protects posterior gut specification by blocking GLD-3/2 polyadenylation of anterior factor neg-1

Project description:The Poly(A)-Tail focused RNA-seq, or PAT-seq approach was utilised to determine the gene expression, poly(A)-site and polyadenylation state of the transcriptome of early C. elegans embryos having been depleated for a series of RNA binding proteins. Namely; pos-1 and mex-5 were independently knocked down using dsRNA expressing E. coli HT115(DE3) fed to ~100,000 starved/synchronized L1 larvae. When half of the population reached adulthood and half were still in the L4 stage, worms were bleached and embryos harvested and stored in Trizol. This ensured an enrichment of early embryos between 1 and 24 cell stage. mex-6(pk440) were synchronized and grown on OP50 until the same time point and embryos harvested in the same manner. Since prolonged gld-3 and gld-2 RNAi result in sterility, starved/synchronized L1 larvae were fed diluted OP50 (200uL of concentrated OP50 diluted in 2mL of M9 and starved L1s) for 16 hours. At that point the OP50 had been mostly consumed by the larvae and concentrated gld-3 or gld-2 dsRNA expressing E. coli were added to the plates. When half of the population reached adulthood and half were still in the L4 stage, worms were bleached and embryos harvested and stored in Trizol. Total RNA was isolated using standard procedures.

2015-05-25 | GSE57993 | GEO

Microarray analysis on fruit fly (Drosophila melanogaster) larvae fed with either a semi-purified diet only or a semi-purified diet supplemented with palm fruit juice (PFJ)

Project description:PFJ (4 ml for a final concentration of 19,000 mg gallic acid equivalent (GAE) per kg diet or 0.86 mg GAE per kcal diet) was supplemented to larvae of fruit flies (Drosophila melanogaster) given a semi-purified diet to observe for possible effects on energy metabolism and lifespan. Larvae were used five days since the egg stage for gene expression studies. Results from the microarray data analysis carried out show that fruit fly larvae given PFJ had up-regulated transport and metabolic processes, while development and morphogenesis processes were down-regulated.

2017-01-06 | GSE93220 | GEO

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

Tweets

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data