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ABSTRACT:
PROVIDER: PRJDB20062 | ENA |
REPOSITORIES: ENA
Similar Datasets
Project description:Analysis of gene expression levels in BBF2H7-/- chondrocytes
| PRJNA119309 | ENA
Project description:Primary outcome(s): Cancer related genome analysis, gene expression and molecular characterization analysis
| 2647002 | ecrin-mdr-crc
Project description:Genome sequencing and gene expression analysis of Protaetia brevitarsis seulensis (KAP220314)
| PRJDB19367 | ENA
Project description:To investigate the altered gene expression levels in macrophages treated with culture medium from apoptotic chondrocytes and normal chondrocytes. Each group had a biological repeat (n=3). Results provide insight into the effect of culture medium from apoptotic chondrocytes on macrophages.
2025-04-27 | GSE201711 | GEO
Project description:Purpose: To reveal the effects of thrombin on the proliferation, morphology, and gene expression in chondrocytes. Methods: Rat primary chondrocytes were cultured with RS, RP and RPT for 24hours, and RNA was extracted for sequencing. The chondrocytes’ morphology was investigated under inverted microscope. Chondrocyte’s proliferation was evaluated by Edu staining, CCK8 methods and PPI network of proliferation-associated genes. The differential gene expression was visualized using a volcano plot. The variance in expression levels of the genes was visualized by the heatmap. GO-based functional annotation clustering analysis was performed on the upregulated and downregulated DEGs, which included biological process, cellular component, and molecular function. The endoplasmic reticulum of chondrocytes from three groups were labeled by molecular probes. The expression levels of Col2a1 and proteoglycan synthesis were detected by cellular immunofluorescence and Safranin O staining separately. The expression levels of Lox were determined by immunocytochemistry staining. The following genes including Comp, Eln, P3H1 and Colgalt1 were examined by RT-qPCR. The expression of MMPs and Sox9 were evaluated by Western Blot. Further analysis of PPI network was performed, and the corresponding modules were selected. Results: As compared with RP group, chondrocytes proliferation was obvious and normal chondrocytes quickly transformed into fibroblast-like chondrocytes in the presence of RS and RPT. we found that 727 genes were up-regulated, and 1162 genes were down-regulated in Venn diagram through analyzing differentially expressed genes (DEGs). The results of GO analyses showed that both the expression of ECM and MMPs were significantly influenced by RS and RPT. Both the labeled endoplasmic reticulum and the expression of representative genes analyzed from the results of RNA sequencing confirmed that chondrocytes were dominated by catabolism. The expression of ECM, MMPs, inflammatory factors and chemokines were verified at the protein level or at the RNA level which agreed with PPI network and functional modules. Conclusions: Thrombin stimulated the proliferation and promoted the morphology transformation towards fibrotic chondrocytes. Thrombin improved the chondrocytes’ catabolism by regulating the expression of the key enzymes which were responsible for ECM synthesis. Meanwhile thrombin increased the expression of MMPs and chemokines that aimed at extracellular ECM degradation.
2024-09-08 | GSE184026 | GEO
Project description:To investigate the altered gene expression levels in chondrocytes cocultured with BMSCs. Each group had a biological repeat (n=3). Results provide insight into the effect of chondrocytes cocultured with bone marrow stem cells
2022-04-27 | GSE191024 | GEO
Project description:Azotobacter vinelandii in response to low nitrogen gas levels (CBX192)
| PRJDB20123 | ENA
Project description:MicroRNAs (miRNAs) regulate cartilage differentiation and contribute to the onset and progression of joint degeneration. These small RNA molecules may affect extracellular matrix organization (ECM) in cartilage, but for only a few miRNAs has this role been defined in vivo. Previously, we showed that cartilage-specific genetic ablation of the Mirc24 cluster in mice leads to impaired cartilage development due to increased RAF/MEK/ERK pathway activation. Here, we studied the expression of the cluster in cartilage by LacZ reporter gene assays and determined its role for extracellular matrix homeostasis by proteome and immunoblot analysis. The cluster is expressed in prehypertrophic/hypertrophic chondrocytes of the growth plate and we now show that the cluster is also highly expressed in articular cartilage. Cartilage-specific loss of the cluster leads to increased proteoglycan 4 and matrix matrix metallopeptidase 13 levels and decreased aggrecan and collagen X levels in epiphyseal cartilage. Interestingly, these changes are linked to a decrease in SRY-related HMG box-containing (SOX) transcription factors 6 and 9, which regulate ECM production in chondrocytes. Our data suggests that the Mirc24 cluster is important for ECM homoeostasis and the expression of transcriptional regulators of matrix production in cartilage.
2020-06-18 | PXD019374 | Pride
Project description:Influence of sex steroids on the pituitary and the adrenal glands assessed by comprehensive gene expression analysis (CBX66)
| PRJDB20043 | ENA