Project description:MEF cells were sequentially infected with H-RasV12 and Twist (Twist1 or Twist2) expression retroviral constructs. Gene expression profiles of the resulting transformed cell lines were compared to the gene expression profile of primary MEF cells
Project description:This study investigated the impact of moderate electric field (MEF) and shear stress (SS) on the chemical profile of a blended fruit and vegetable juice using untargeted metabolomics. Samples underwent mild thermal treatment, MEF, and a combination of MEF and SS, with subsequent chemical changes analyzed through liquid chromatography-mass spectrometry (LC-MS). Principal component analysis revealed distinct clustering based on treatment type, indicating that processing with MEF and MEF+SS altered the juice metabolite composition compared to both fresh juice and sample subjected to mild thermal treatment. Notably, anthocyanins underwent degradation, with MEF and MEF+SS treatments resulting in increased rates of compound breakdown and/or oxidation. Furthermore, MEF and MEF+SS treated samples had higher levels of some phenolic acids, such as gallic acid and caffeic acid, suggesting enhanced bioaccessibility and bioavailability. These findings suggest that MEF, both with and without SS, induces significant non-thermal effects, contributing to the degradation or release of key metabolites. This work underscores the potential of MEF and MEF+SS technologies to modify food composition during processing, offering new opportunities for optimizing food quality.
