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Gut metagenome

ABSTRACT: Effects of compost-derived thermophile administration on heat stress mortality in chickens

PROVIDER: PRJDB20309 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	DRR685878_1.fastq.gz	Fastqsanger.gz
	DRR685878_2.fastq.gz	Fastqsanger.gz
	DRR685879_1.fastq.gz	Fastqsanger.gz
	DRR685879_2.fastq.gz	Fastqsanger.gz
	DRR685880_1.fastq.gz	Fastqsanger.gz

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Project description:To realize the gene expression in response to acute heat stress in chicken testis, we have employed whole genome microarray expression profiling as we have employed whole genome microarray expression profiling as a tool to identify genes response to acute heat stress. Male B strain Taiwan country chickens were subjected to acute heat stress (38℃) for 4 h, and then exposed to 25℃, with testes collected 0, 2, and 6 h after the cessation of heat stress, using non heat-stressed roosters as a control group (n = 3 roosters per group). Based on a chicken 44K oligo microarray, 163 genes significantly differed in the testes of the heat-stressed chickens from those of the control chickens. The mRNA expressions of upregulated genes, including HSP25, HSP90AA1, HSPA2, and LPAR2, and downregulated genes, including CDH5, CTNNA3, EHF, CIRBP, SLA, and NTF3, were confirmed through quantitative real-time polymerase chain reaction (qRT-PCR).

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